Fibroblast growth factor-1-inducible gene FR-17 encodes a nonmuscle alpha-actinin isoform

Journal of Cellular Physiology
D K HsuJ A Winkles

Abstract

Polypeptide growth factor binding to cell surface receptors activates a cytoplasmic signaling cascade that ultimately promotes the expression of specific nuclear genes. As an approach to investigate the molecular mechanism of fibroblast growth factor (FGF)-1 mitogenic signaling, we have begun to identify and characterize FGF-1-inducible genes in murine NIH 3T3 cells. Here we report that one of these genes, termed FGF-regulated (FR)-17, is predicted to encode a nonmuscle isoform of alpha-actinin, an actin cross-linking protein found along microfilaments and in focal adhesion plaques. FGF-1 induction of alpha-actinin mRNA expression is first detectable at 2 h after mitogen addition and is dependent on the novo RNA and protein synthesis. Maximal alpha-actinin mRNA expression, corresponding to an approximately nineteenfold level of induction, is present after 12 h of FGF-1 stimulation. Western blot analysis indicated that FGF-1-stimulated cells also produce an increased amount of alpha-actinin protein. The FGF-1-related mitogen FGF-2, calf serum, several of the polypeptide growth factors present in serum, and the tumor promoter phorbol myristate acetate can also induce alpha-actinin mRNA expression. Finally, nonmuscle alpha-actinin...Continue Reading

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Citations

Apr 29, 1998·The Journal of Cell Biology·K HondaS Hirohashi
Jul 27, 2006·Arthritis and Rheumatism·Yves RenaudineauPierre Youinou
Mar 9, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Bisram DeocharanChaim Putterman
Jun 6, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Zeguo ZhaoChaim Putterman
Feb 16, 2000·Biochemical and Biophysical Research Communications·A E El-HusseiniS R Vincent

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