Fibroblast growth factor-2 regulates expression of osteopontin in periodontal ligament cells

Journal of Cellular Physiology
Yoshimitsu TerashimaShinya Murakami

Abstract

Osteopontin is a protein found in the bone-related matrix and plays multiple regulatory roles in mineralizing and non-mineralizing tissue. In osteogenic cell-lines, the expression of osteopontin increases with the progression of differentiation, but both the expression and function of osteopontin vary with the cell type and its activation state. In this study, we examined the expression of osteopontin by clones established from mouse periodontal ligament, in response to inorganic phosphate and fibroblast growth factor (FGF)-2, which can induce periodontal tissue regeneration. The involvement of inorganic phosphate in the expression of osteopontin during the course of cell differentiation of a clone MPDL22 was confirmed by addition of foscarnet, an inorganic phosphate transport inhibitor. Although FGF-2 decreased the mRNA expression of almost every bone-related protein in MPDL22, FGF-2 upregulated the expression of osteopontin in MPDL22 at both mRNA and protein levels. Interestingly, FGF-2 enhanced the concentration of osteopontin in the culture supernatant of MPDL22, whereas inorganic phosphate did not. The FGF-2-induced osteopontin in the culture supernatant seems to be involved in cell survival activity. An immunohistochemica...Continue Reading

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Citations

Jul 16, 2014·Journal of Indian Society of Periodontology·Sameera G Nath, Ranjith Raveendran
Jan 26, 2016·Matrix Biology : Journal of the International Society for Matrix Biology·Timothy N Trotter, Yang Yang
Oct 17, 2012·The Journal of Evidence-based Dental Practice·Mark A Reynolds, Mary E Aichelmann-Reidy
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Apr 20, 2011·Periodontology 2000·Yuichi IzumiIsao Ishikawa
May 8, 2010·Journal of Clinical Periodontology·Yoshinori ShirakataKazuyuki Noguchi
May 2, 2015·Journal of Clinical Periodontology·Ronaldo Barcellos de Santana, Carolina Miller Mattos de Santana
Feb 24, 2015·Dental Materials : Official Publication of the Academy of Dental Materials·Sophia P PilipchukWilliam V Giannobile
Sep 25, 2015·Journal of Dental Research·S YamabaS Murakami
Nov 5, 2013·Journal of Dental Research·M YanagitaS Murakami
Nov 7, 2020·Current Drug Targets·Hamideh Sadat MohammadipourAli Forouzanfar

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