Fibroblast growth factor-23 induces cellular senescence in human mesenchymal stem cells from skeletal muscle

Biochemical and Biophysical Research Communications
Chisato SatoHiroshi Suzuki

Abstract

Although muscle wasting and/or degeneration are prevalent in patients with chronic kidney disease, it remains unknown whether FGF-23 influences muscle homeostasis and regeneration. Mesenchymal stem cells (MSCs) in skeletal muscle are distinct from satellite cells and have a known association with muscle degeneration. In this study we sought to investigate the effects of FGF-23 on MSCs isolated from human skeletal muscle in vitro. The MSCs expressed FGF receptors (1 through 4) and angiotensin-II type 1 receptor, but no traces of the Klotho gene were detected. MSCs and satellite cells were treated with FGF-23 and angiotensin-II for 48 h. Treatment with FGF-23 significantly decreased the number of MSCs compared to controls, while treatment with angiotensin-II did not. FGF-23 and angiotensin-II both left the cell counts of the satellite cells unchanged. The FGF-23-treated MSCs exhibited the senescent phenotype, as judged by senescence-associated β-galactosidase assay, cell morphology, and increased expression of p53 and p21 in western blot analysis. FGF-23 also significantly altered the gene expression of oxidative stress regulators in the cells. In conclusion, FGF-23 induced premature senescence in MSCs from skeletal muscle via th...Continue Reading

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Citations

Jul 9, 2016·Radiation Research·Heather A HimburgJohn P Chute
May 4, 2017·Clinical and Experimental Pharmacology & Physiology·Yosuke HirakawaReiko Inagi
May 16, 2020·Calcified Tissue International·Gemma Marcucci, Maria Luisa Brandi
Feb 28, 2019·Orphanet Journal of Rare Diseases·Signe Sparre Beck-NielsenOuti Mäkitie
Jun 23, 2019·GeroScience·Christy S CarterLaDora Thompson
Feb 23, 2019·International Journal of Molecular Sciences·Deborah MattinzoliPiergiorgio Messa
Jun 10, 2021·Scientific Reports·Ying SiPeter H King

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