Fibroblast growth factor and heparin protect endothelial cells from the effects of interleukin 1

Journal of Cellular Physiology
A J MinterJ Dawes

Abstract

Vascular endothelium is involved in both active and passive processes in haemostasis, but inflammatory cytokines such as interleukin 1 (IL-1) and tumour necrosis factor (TNF) have been reported to convert the comparatively inert endothelial cell to an inflammatory state. Acidic fibroblast growth factor (aFGF) in the presence of heparin has effects opposite to IL-1 on cultured human umbilical vein endothelial cells (HUVEC); therefore, we have investigated the modulation of IL-1-induced effects by the c combination of aFGF and heparin (aFGF/heparin). First passage HUVEC were cultured for 6 days in the presence of 20% human serum with and without the addition of 625 pM human recombinant aFGF (hr aFGF) and 7 microM heparin. On day 5, recombinant IL-1 beta was included for 24 h. The following day the cells were washed and measurements made of the release of prostacyclin, von Willebrand factor, plasminogen activator inhibitor type 1, and thrombospondin, both in the resting state and following stimulation for 60 min with 1 U/ml thrombin. Tissue-type plasminogen activator was assayed in HUVEC lysates. Similar experiments were performed to assess effects on the expression of vascular adhesion molecule, intracellular adhesion molecule, a...Continue Reading

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Citations

Jul 1, 1997·Inflammatory Bowel Diseases·J R Korzenik
Dec 10, 2008·Endothelium : Journal of Endothelial Cell Research·N BerghS Jern
Jun 21, 2007·Endothelium : Journal of Endothelial Cell Research·Richard DaculsiLaurence Bordenave
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Aug 5, 2000·Biochemical and Biophysical Research Communications·D Zapolska-DownarM Naruszewicz

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