Fibroblast nemosis induces angiogenic responses of endothelial cells

Experimental Cell Research
Anna EnzerinkAntti Vaheri

Abstract

Increasing evidence points to a central link between inflammation and activation of the stroma, especially of fibroblasts therein. However, the mechanisms leading to such activation mostly remain undescribed. We have previously characterized a novel type of fibroblast activation (nemosis) where clustered fibroblasts upregulated the production of cyclooxygenase-2, secretion of prostaglandins, proteinases, chemotactic cytokines, and hepatocyte growth factor (HGF), and displayed activated nuclear factor-kappaB. Now we show that nemosis drives angiogenic responses of endothelial cells. In addition to HGF, nemotic fibroblasts secreted vascular endothelial growth factor (VEGF), and conditioned medium from spheroids promoted sprouting and networking of human umbilical venous endothelial cells (HUVEC). The response was partly inhibited by function-blocking antibodies against HGF and VEGF. Conditioned nemotic fibroblast medium promoted closure of HUVEC and human dermal microvascular endothelial cell monolayer wounds, by increasing the motility of the endothelial cells. Wound closure in HUVEC cells was partly inhibited by the antibodies against HGF. The stromal microenvironment regulates wound healing responses and often promotes tumorig...Continue Reading

References

Aug 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·K M WeidnerY Daikuhara
Sep 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·T NakamuraA Ichihara
Feb 1, 1971·The Journal of Experimental Medicine·J FolkmanG Williams
Feb 15, 1994·International Journal of Cancer. Journal International Du Cancer·M T Dimanche-BoitrelF Martin
May 24, 1994·Proceedings of the National Academy of Sciences of the United States of America·S RongG F Vande Woude
Nov 14, 1997·Nature Medicine·M SkobeN E Fusenig
Jun 5, 2003·Nature Medicine·Napoleone FerraraJennifer LeCouter
Oct 14, 2003·Cell Death and Differentiation·J BizikA Vaheri
Dec 20, 2003·Nature Reviews. Molecular Cell Biology·Carmen BirchmeierGeorge F Vande Woude
Dec 23, 2003·Proceedings of the National Academy of Sciences of the United States of America·Sung-Hee ChangTimothy Hla
Feb 27, 2004·Biochimica Et Biophysica Acta·Jane Sottile
Nov 19, 2004·Nature·Neil A BhowmickHarold L Moses
Nov 24, 2004·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Patrick Micke, Arne Ostman
Mar 18, 2005·Nature·Carla BoccaccioPaolo M Comoglio
May 4, 2005·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Wanzhong WangJan-Erik Damber
Jul 30, 2005·The EMBO Journal·Kentaro KajiyaMichael Detmar
Mar 31, 2006·Nature Reviews. Cancer·Raghu Kalluri, Michael Zeisberg
Aug 2, 2006·Cell Cycle·Akira Orimo, Robert A Weinberg
Aug 19, 2006·Immunological Investigations·Carolyn J BagloleRichard P Phipps
Feb 6, 2007·Clinics in Dermatology·Jie LiRobert Kirsner
Feb 15, 2007·The Journal of Investigative Dermatology·Sabine A EmingJeffrey M Davidson
Feb 15, 2007·The Journal of Investigative Dermatology·Boris Hinz
Apr 4, 2007·The Journal of Cell Biology·Jolanta ChmielowiecWalter Birchmeier
May 25, 2007·Nature Reviews. Molecular Cell Biology·Ralf H Adams, Kari Alitalo
Nov 6, 2007·Progress in Histochemistry and Cytochemistry·Sabine A EmingManuel Koch
Dec 15, 2007·Cardiovascular Research·Victor W M van Hinsbergh, Pieter Koolwijk
Feb 20, 2008·Angiogenesis·Sebastien P Tabruyn, Arjan W Griffioen
Apr 9, 2008·Current Opinion in Hematology·Christopher C W Hughes
Jul 17, 2008·Nature Reviews. Molecular Cell Biology·Matthias Schäfer, Sabine Werner
Aug 19, 2008·Biochemical Pharmacology·Paola Chiarugi, Elisa Giannoni
Sep 9, 2008·International Journal of Cancer. Journal International Du Cancer·Olivier De WeverMarc Bracke
Oct 1, 2008·Experimental Cell Research·Pertteli SalmenperäAntti Vaheri
Mar 21, 2009·Experimental Cell Research·Antti VaheriPertteli Salmenperä
May 2, 2009·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Rana ZeineSusan L Cohn
Jul 21, 2009·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Matti PeuraEsko Kankuri

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Citations

May 11, 2010·Experimental Cell Research·Kati Räsänen, Antti Vaheri
Jan 25, 2011·Journal of Thrombosis and Haemostasis : JTH·A Enzerink, A Vaheri
Feb 2, 2013·The Journal of Surgical Research·Dingzhi LiuLong Pan
Nov 25, 2011·Burns : Journal of the International Society for Burn Injuries·Matti PeuraJyrki Vuola
Aug 16, 2014·Frontiers in Bioengineering and Biotechnology·Ville RantanenSampsa Hautaniemi
Dec 25, 2019·Biochemical and Biophysical Research Communications·Mona MehrubaHideyuki Mukai

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