PMID: 2504659Aug 1, 1989Paper

Fibroblast-specific common fragile sites induced by aphidicolin

Human Genetics
I MuranoT Kajii

Abstract

The distribution and frequency of aphidicolin-induced common fragile sites were studied in chromosomes of cultured skin fibroblasts and PHA-stimulated lymphocytes from five normal individuals; 0.2 microM aphidicolin was added for the last 26 h of culture. Skin fibroblasts from five fra(X)-positive patients were also studied in the same manner. Fragile sites most frequently found in fibroblasts from normal individuals were 3q26.2, 7q11.23, 16q23, 1p31, 10q11.2, 12q23 and 7q31, whereas those in lymphocytes from the same individuals were 3p14, 16q23, Xp22, 7q32 and 14q24. The distribution of fragile sites in fibroblasts from fra(X)-positive patients was essentially identical with that in normal individuals. The average number of gaps and breaks in 100 metaphases was 36.8 in fibroblasts from normal individuals, 113.8 in those from fra(X)-positive patients, and 279 in lymphocytes from normal individuals. Their rates of chromosome-type breaks and gaps were 7.9%, 29.7% and 54.5%, respectively. Thus, the distribution and frequency of aphidicolin-induced fragile sites were different between skin fibroblasts and lymphocytes, possibly reflecting differences in their DNA replication sequence or gene activity.

References

Sep 1, 1988·Jinrui idengaku zasshi. The Japanese journal of human genetics·A KuwanoT Kajii
Mar 1, 1988·Cancer Genetics and Cytogenetics·R MorganF Hecht
Jan 1, 1987·Cytogenetics and Cell Genetics·G R Sutherland, J F Mattei

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Citations

Apr 1, 1996·Human Genetics·I Simonic, G S Gericke
Jan 1, 1995·Biulleten' eksperimental'noĭ biologii i meditsiny·S V Stukalov
Aug 1, 1992·Cancer Genetics and Cytogenetics·R A BeckerS L Wenger
Jun 28, 2013·PloS One·Megha RajaramR Scott Powers
Sep 23, 2014·Cellular and Molecular Life Sciences : CMLS·Alexandros G GeorgakilasVassilis G Gorgoulis
Sep 25, 2014·Cellular and Molecular Life Sciences : CMLS·Benoît Le TallecMichelle Debatisse
Nov 19, 2011·Trends in Genetics : TIG·Michelle DebatisseOlivier Brison
Jun 20, 2013·Genes, Chromosomes & Cancer·Elisa PalumboAntonella Russo
Aug 26, 1998·The Journal of Veterinary Medical Science·K KuboY Takamori
Jan 18, 2015·Experimental Biology and Medicine·Ge Gao, David I Smith
Jul 26, 2017·Nature Reviews. Cancer·Thomas W GloverMartin F Arlt
Apr 12, 2016·Current Opinion in Cell Biology·Dan Sarni, Batsheva Kerem
Nov 6, 2018·Genes, Chromosomes & Cancer·Elisa Palumbo, Antonella Russo
Dec 14, 2018·Genes, Chromosomes & Cancer·Michal Irony-Tur Sinai, Batsheva Kerem
Aug 15, 2019·Nucleic Acids Research·Michal Irony-Tur SinaiBatsheva Kerem
Sep 24, 2020·Cell Reports·Arijita ChakrabortyWenyi Feng

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