PMID: 8445407Feb 1, 1993Paper

Fibroblasts are required for Schwann cell basal lamina deposition and ensheathment of unmyelinated sympathetic neurites in culture

Journal of Neurocytology
V J ObremskiM B Bunge

Abstract

The ability to purify and recombine populations of peripheral neurons, Schwann cells and fibroblasts in tissue culture has enabled us to examine the contribution of fibroblasts to Schwann cell basal lamina assembly and ensheathment of unmyelinated rat superior cervical ganglion neurites in vitro. Purified perinatal superior cervical ganglion neurons were grown in culture either with Schwann cells or with Schwann cells plus fibroblasts derived from either superior cervical ganglion capsule or cranial periosteum. The cultures were maintained for 2-8 weeks on a collagen substratum in a medium known to promote Schwann cell differentiation (myelin, basal lamina formation) in the presence of dorsal root ganglion neurons. The extent of Schwann cell differentiation (ensheathment, basal lamina formation) in the presence of superior cervical ganglion neurons was evaluated in this study using electron microscopy. In superior cervical ganglion neuron plus Schwann cell cultures (without fibroblasts), Schwann cells achieved only a moderate degree of ensheathment; also, Schwann cell basal lamina was discontinuous and extracellular collagen fibrils were sparse. Although only discontinuous basal lamina was demonstrable by electron microscopy in...Continue Reading

References

Jan 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·J E Bottenstein, G H Sato
Jan 1, 1986·Developmental Neuroscience·A Baron-Van EvercoorenH K Kleinman
Jun 1, 1986·The Journal of Cell Biology·D J CareyC M Rafferty
Mar 1, 1988·The Journal of Investigative Dermatology·P DelvoyeC M Lapiere
Jan 1, 1986·Annual Review of Neuroscience·R P BungeC F Eldridge
Jan 1, 1986·Annals of the New York Academy of Sciences·R Mirsky, K R Jessen
Mar 1, 1986·The Journal of Cell Biology·R D SandersonR Mayne
Jan 1, 1984·Differentiation; Research in Biological Diversity·U KühlK von der Mark
Aug 1, 1983·The Journal of Cell Biology·D J CareyR P Bunge
Nov 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·F MoyaR P Bunge

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Citations

Jan 25, 2002·The Journal of Comparative Neurology·Karen L LankfordJeffery D Kocsis
Jul 30, 2009·Cell and Tissue Research·Yujun WeiXiufang Zhang
Oct 1, 1993·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·R J Franklin, W F Blakemore
Jan 1, 1997·Journal of Anatomy·S Hall
Dec 6, 2008·Genes & Development·Simona ParrinelloAlison C Lloyd
Dec 10, 1999·Annals of the New York Academy of Sciences·T RosenbaumN Ratner
Sep 1, 2005·Neuron·Carla TaveggiaJames L Salzer
May 21, 1999·The European Journal of Neuroscience·C H ChauK F So
Mar 15, 1996·Journal of Neuroscience Research·L MinghettiP Stroobant
Mar 6, 2003·The Anatomical Record. Part B, the New Anatomist·Robin J M Franklin
Oct 1, 1999·Annals of the New York Academy of Sciences·Thorsten RosenbaumNancy Ratner
Feb 18, 2021·Tissue Engineering. Part B, Reviews·Omar A SelimDeepak M Kalaskar
Jan 29, 1996·The Journal of Comparative Neurology·P G Smith, Q Fan
Jun 17, 2021·Journal of Anatomy·Chelsey B ReedEmma R Wilson

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