Fibroblasts from bank voles inhabiting Chernobyl have increased resistance against oxidative and DNA stresses

BMC Cell Biology
Venla MustonenJaana Jurvansuu

Abstract

Elevated levels of environmental ionizing radiation can be a selective pressure for wildlife by producing reactive oxygen species and DNA damage. However, the underlying molecular mechanisms that are affected are not known. We isolated skin fibroblasts from bank voles (Myodes glareolus) inhabiting the Chernobyl nuclear power plant accident site where background radiation levels are about 100 times greater than in uncontaminated areas. After a 10 Gy dose of gamma radiation fibroblasts from Chernobyl animals recovered faster than fibroblasts isolated from bank voles living in uncontaminated control area. The Chernobyl fibroblasts were able to sustain significantly higher doses of an oxidant and they had, on average, a higher total antioxidant capacity than the control fibroblasts. Furthermore, the Chernobyl fibroblasts were also significantly more resistant than the control fibroblasts to continuous exposure to three DNA damaging drugs. After drug treatment transcription of p53-target gene pro-apoptotic Bax was higher in the control than in the Chernobyl fibroblasts. Fibroblasts isolated from bank voles inhabiting Chernobyl nuclear power plant accident site show elevated antioxidant levels, lower sensitivity to apoptosis, and inc...Continue Reading

References

Feb 1, 1986·Japanese Journal of Pharmacology·K Sugihara, M Gemba
Jun 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·J M Lee, A Bernstein
Nov 25, 1998·Proceedings of the National Academy of Sciences of the United States of America·H K ShuM A Israel
Jan 30, 2004·Journal of Environmental Radioactivity·Ronald K ChesserBrenda E Rodgers
May 11, 2004·Plant Physiology·Igor KovalchukOlga Kovalchuk
Aug 11, 2004·Proceedings of the National Academy of Sciences of the United States of America·Osnat Ashur-FabianGideon Rechavi
Feb 12, 2005·Proceedings. Biological Sciences·A P MøllerT A Mousseau
Jul 25, 2006·Radiation and Environmental Biophysics·Nadezhda I Ryabokon, R I Goncharova
Feb 1, 2008·Environment International·S A Geras'kinR M Alexakhin
Apr 9, 2008·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Anders Pape MøllerTimothy A Mousseau
Dec 17, 2009·Annals of the New York Academy of Sciences·Alexey V YablokovAlexey V Nesterenko
Feb 6, 2013·Cancer Biology & Therapy·Derek Woods, John J Turchi
Feb 11, 2015·Scientific Reports·Anders Pape Møller, Timothy A Mousseau
Oct 7, 2015·Current Biology : CB·T G DeryabinaJ T Smith
Feb 7, 2016·The Science of the Total Environment·D EinorA P Møller
Feb 13, 2016·Trends in Ecology & Evolution·Anders Pape Møller, Timothy Alexander Mousseau
Nov 21, 2017·Evolutionary Applications·Robert J BakerCaleb D Phillips

❮ Previous
Next ❯

Citations

Jul 15, 2020·International Journal of Radiation Biology·Georgetta Cannon, Juliann G Kiang
May 10, 2020·Evidence-based Complementary and Alternative Medicine : ECAM·Chengxu MaYingdong Li
Sep 17, 2020·International Journal of Molecular Sciences·Usha KabilanDmitry Klokov
Jan 20, 2022·Antioxidants & Redox Signaling·Jaakko L O Pohjoismäki, Steffi Goffart

❮ Previous
Next ❯

Methods Mentioned

BETA
flow cytometry
Assay
Protein Assay
transfection
PCR

Software Mentioned

R
Flowing

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis