Abstract
The nuclear lamina, an intermediate filament meshwork lining the inner nuclear membrane, is formed by the nuclear lamins (lamins A, C, B1, and B2). Defects or deficiencies in individual nuclear lamin proteins have been reported to elicit nuclear blebs (protrusions or outpouchings of the nuclear envelope) and increase susceptibility for nuclear membrane ruptures. It is unclear, however, how a complete absence of nuclear lamins would affect nuclear envelope morphology and nuclear membrane integrity (i.e., whether nuclear membrane blebs or protrusions would occur and, if not, whether cells would be susceptible to nuclear membrane ruptures). To address these issues, we generated mouse embryonic fibroblasts (MEFs) lacking all nuclear lamins. The nuclear lamin-deficient MEFs had irregular nuclear shapes but no nuclear blebs or protrusions. Despite a virtual absence of nuclear blebs, MEFs lacking nuclear lamins had frequent, prolonged, and occasionally nonhealing nuclear membrane ruptures. By transmission electron microscopy, the inner nuclear membrane in nuclear lamin-deficient MEFs have a "wavy" appearance, and there were discrete discontinuities in the inner and outer nuclear membranes. Nuclear membrane ruptures were accompanied by...Continue Reading
References
Jun 1, 1994·The Journal of Cell Biology·R D MoirR D Goldman
May 12, 2000·Journal of Structural Biology·R D MoirR D Goldman
Nov 3, 2001·Science·C M de NoronhaW C Greene
Apr 26, 2003·Nature·Maria ErikssonFrancis S Collins
Jul 3, 2004·Proceedings of the National Academy of Sciences of the United States of America·Laurent VergnesKaren Reue
Dec 21, 2004·Proceedings of the National Academy of Sciences of the United States of America·Loren G FongStephen G Young
Mar 18, 2006·Science·Ming-Ying TsaiYixian Zheng
Apr 9, 2008·Experimental Cell Research·P J Stewart-HutchinsonDidier Hodzic
Jul 10, 2009·The Journal of Clinical Investigation·Howard J WormanStephen G Young
Feb 11, 2010·Proceedings of the National Academy of Sciences of the United States of America·Catherine CoffinierStephen G Young
Jun 11, 2011·Human Molecular Genetics·Shao H YangStephen G Young
Aug 13, 2011·Human Molecular Genetics·Winnok H De VosJos L V Broers
Oct 7, 2011·Molecular Biology of the Cell·Catherine CoffinierStephen G Young
Mar 15, 2012·The Journal of Biological Chemistry·Stephen G YoungLoren G Fong
May 9, 2012·Nucleus·Jesse D VargasMartin W Hetzer
Dec 6, 2012·Nature Reviews. Molecular Cell Biology·Brian Burke, Colin L Stewart
Feb 13, 2013·Proceedings of the National Academy of Sciences of the United States of America·Chloe M FunkhouserMonica Olvera de la Cruz
May 8, 2013·Proceedings of the National Academy of Sciences of the United States of America·Hea-Jin JungStephen G Young
Jul 6, 2013·Cell·Emily M HatchMartin W Hetzer
Aug 28, 2013·Cell Research·Youngjo KimYixian Zheng
Feb 20, 2014·Genesis : the Journal of Genetics and Development·David Razafsky, Didier Hodzic
Apr 23, 2014·The Journal of Cell Biology·Emily Hatch, Martin Hetzer
Oct 15, 2014·Molecular and Cellular Biology·Hea-Jin JungStephen G Young
Aug 8, 2015·Molecular Biology of the Cell·Yuxuan Guo, Yixian Zheng
Feb 20, 2016·Current Opinion in Cell Biology·Alexandra Lynn McGregorJan Lammerding
Mar 26, 2016·Science·M RaabM Piel
Mar 26, 2016·Science·Celine M DenaisJan Lammerding
Oct 5, 2016·The Journal of Cell Biology·Emily M Hatch, Martin W Hetzer
Oct 5, 2016·The Journal of Cell Biology·Jan Lammerding, Katarina Wolf
Mar 14, 2017·Nucleus·Philipp Isermann, Jan Lammerding
Feb 21, 2018·Current Opinion in Cell Biology·Emily M Hatch
Mar 2, 2018·Circulation·Magda R HamczykVicente Andrés
Sep 28, 2018·Science Translational Medicine·Paul H KimLoren G Fong
Citations
Apr 23, 2019·Journal of Cellular Physiology·Ian A KentTanmay P Lele
May 31, 2019·The Journal of Cell Biology·Charles T HalfmannKyle J Roux
Dec 5, 2019·Proceedings of the National Academy of Sciences of the United States of America·Natalie Y ChenStephen G Young
Jun 25, 2019·Journal of Biomechanical Engineering·Richard D H TranAnna Grosberg
Aug 4, 2020·Advanced Healthcare Materials·Christopher M MadlSarah C Heilshorn
Jul 12, 2019·Frontiers in Cell and Developmental Biology·Navjot Kaur GillAmy C Rowat
May 3, 2020·Proceedings of the National Academy of Sciences of the United States of America·Jason N BellingSteven J Jonas
Jul 17, 2020·Chromosoma·Xihan GuoXu Wang
May 1, 2020·Cell Death Discovery·Liora LindenboimReuven Stein
Mar 14, 2019·Cells·Jungwon Hah, Dong-Hwee Kim
Jul 8, 2019·GeroScience·Susana Gonzalo, Nuria Coll-Bonfill
Feb 16, 2019·Proceedings of the National Academy of Sciences of the United States of America·Bruce NmeziQuasar S Padiath
Aug 18, 2020·The FEBS Journal·Agathe MarcelotSophie Zinn-Justin
Aug 21, 2020·Journal of Cell Science·Rhiannon M Sears, Kyle J Roux
Sep 11, 2020·Nucleus·Natalie Y ChenStephen G Young
Jul 22, 2020·Annual Review of Cell and Developmental Biology·John Maciejowski, Emily M Hatch
Oct 8, 2020·Cell Death Discovery·Liora LindenboimReuven Stein
Feb 4, 2021·Nucleic Acids Research·Laura A Murray-NergerIleana M Cristea
Mar 9, 2021·Cell Cycle·Charles T Halfmann, Kyle J Roux
Feb 11, 2021·Cell Death Discovery·Liora LindenboimReuven Stein
Mar 20, 2019·Current Opinion in Cell Biology·Andrew D StephensJohn F Marko
Apr 4, 2021·International Journal of Molecular Sciences·Ália Dos Santos, Christopher P Toseland
Apr 12, 2021·Seminars in Cell & Developmental Biology·Anna E Mammel, Emily M Hatch
Jun 22, 2021·Trends in Biochemical Sciences·Laura A Murray-Nerger, Ileana M Cristea
Jun 24, 2021·Proceedings of the National Academy of Sciences of the United States of America·Natalie Y ChenLoren G Fong
Aug 28, 2021·Cells·K Tanuj Sapra, Ohad Medalia
Jul 7, 2021·MBio·Santiago VidalCarmen Rivas
Jul 3, 2021·Annual Review of Cell and Developmental Biology·Philipp Niethammer