Fibroblasts lacking nuclear lamins do not have nuclear blebs or protrusions but nevertheless have frequent nuclear membrane ruptures

Proceedings of the National Academy of Sciences of the United States of America
Natalie Y ChenStephen G Young

Abstract

The nuclear lamina, an intermediate filament meshwork lining the inner nuclear membrane, is formed by the nuclear lamins (lamins A, C, B1, and B2). Defects or deficiencies in individual nuclear lamin proteins have been reported to elicit nuclear blebs (protrusions or outpouchings of the nuclear envelope) and increase susceptibility for nuclear membrane ruptures. It is unclear, however, how a complete absence of nuclear lamins would affect nuclear envelope morphology and nuclear membrane integrity (i.e., whether nuclear membrane blebs or protrusions would occur and, if not, whether cells would be susceptible to nuclear membrane ruptures). To address these issues, we generated mouse embryonic fibroblasts (MEFs) lacking all nuclear lamins. The nuclear lamin-deficient MEFs had irregular nuclear shapes but no nuclear blebs or protrusions. Despite a virtual absence of nuclear blebs, MEFs lacking nuclear lamins had frequent, prolonged, and occasionally nonhealing nuclear membrane ruptures. By transmission electron microscopy, the inner nuclear membrane in nuclear lamin-deficient MEFs have a "wavy" appearance, and there were discrete discontinuities in the inner and outer nuclear membranes. Nuclear membrane ruptures were accompanied by...Continue Reading

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Citations

Apr 23, 2019·Journal of Cellular Physiology·Ian A KentTanmay P Lele
May 31, 2019·The Journal of Cell Biology·Charles T HalfmannKyle J Roux
Dec 5, 2019·Proceedings of the National Academy of Sciences of the United States of America·Natalie Y ChenStephen G Young
Jun 25, 2019·Journal of Biomechanical Engineering·Richard D H TranAnna Grosberg
Jul 12, 2019·Frontiers in Cell and Developmental Biology·Navjot Kaur GillAmy C Rowat
May 3, 2020·Proceedings of the National Academy of Sciences of the United States of America·Jason N BellingSteven J Jonas
May 1, 2020·Cell Death Discovery·Liora LindenboimReuven Stein
Mar 14, 2019·Cells·Jungwon Hah, Dong-Hwee Kim
Jul 8, 2019·GeroScience·Susana Gonzalo, Nuria Coll-Bonfill
Feb 16, 2019·Proceedings of the National Academy of Sciences of the United States of America·Bruce NmeziQuasar S Padiath
Aug 18, 2020·The FEBS Journal·Agathe MarcelotSophie Zinn-Justin
Aug 21, 2020·Journal of Cell Science·Rhiannon M Sears, Kyle J Roux
Jul 22, 2020·Annual Review of Cell and Developmental Biology·John Maciejowski, Emily M Hatch
Feb 4, 2021·Nucleic Acids Research·Laura A Murray-NergerIleana M Cristea
Mar 9, 2021·Cell Cycle·Charles T Halfmann, Kyle J Roux
Feb 11, 2021·Cell Death Discovery·Liora LindenboimReuven Stein
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Jun 22, 2021·Trends in Biochemical Sciences·Laura A Murray-Nerger, Ileana M Cristea
Jun 24, 2021·Proceedings of the National Academy of Sciences of the United States of America·Natalie Y ChenLoren G Fong
Jul 3, 2021·Annual Review of Cell and Developmental Biology·Philipp Niethammer

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