Fibroblasts prevent apoptosis of IL-2-deprived T cells without inducing proliferation: a selective effect on Bcl-XL expression

Immunology
W GombertA N Akbar

Abstract

The apoptosis of human cytokine-deprived activated T cells can be prevented by a soluble mediator secreted by fibroblasts, epithelial and endothelial cells, and this rescue occurs with fibroblasts from different species. Fractionation of W138 fibroblast-conditioned medium indicated that the survival-promoting agent(s) were > 30,000 MW. The continuous presence of the survival factor was required for prevention of apoptosis, which did not involve the induction of proliferation. Nevertheless, the co-cultured T cells remained in a primed state. The expression of the apoptosis-inducing proteins Bax and CD95 (Fas/Apo-1) was either unchanged or slightly increased in fibroblast-rescued T cells, suggesting that constraints on survival still existed after co-culture. A fundamental observation in the present study was that although Bcl-2 was reduced, the levels of Bcl-XL was maintained in cytokine-deprived T cells by fibroblast co-culture. This suggests that fibroblasts and/or other stromal cells may promote activated T-cell survival by a selective effect on Bcl-XL expression, which is consistent with histological examination of activated T cells within lymphoid tissue in vivo. The rescued T cell could be re-activated by CD3 antibody, but...Continue Reading

References

Aug 1, 1992·Immunology Today·S J Korsmeyer
Jun 1, 1991·Immunology Today·A N AkbarG Janossy
Nov 8, 1990·Nature·E B Bell, S M Sparshott
Nov 1, 1990·British Journal of Plastic Surgery·C W Martin, I F Muir
Dec 1, 1990·The Journal of Experimental Medicine·S ScottJ T Kurnick
Nov 1, 1981·The Journal of Cell Biology·T MaciagR Weinstein
Aug 1, 1994·Current Opinion in Immunology·L K Selin, R M Welsh
Feb 2, 1995·Nature·J DheinP H Krammer
Sep 1, 1995·The Journal of Experimental Medicine·D T ChaoS J Korsmeyer
Oct 21, 1994·Cell·Z N Oltvai, S J Korsmeyer
Dec 1, 1994·International Archives of Allergy and Immunology·A N AkbarG Janossy
Apr 1, 1994·Current Opinion in Immunology·P H KrammerK M Debatin
Oct 1, 1993·Journal of Pediatric Surgery·V R AdolphT M Krummel
Jan 28, 1994·Cell·J Sprent

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Citations

Oct 13, 2000·Journal of Cellular Biochemistry·A KehlenD Riemann
Dec 22, 1999·Journal of Hepatology·S M CruickshankL K Trejdosiewicz
Aug 19, 2003·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Bonnie BidingerRobert B Zurier
May 22, 2001·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·M Conron, R M Du Bois
Nov 17, 1999·Scandinavian Journal of Immunology·K SakataH Dang
Feb 24, 2004·The British Journal of Ophthalmology·J G CrowstonA N Akbar
May 23, 1998·Annual Review of Immunology·R W DuttonS L Swain
Feb 1, 1997·The Journal of Clinical Investigation·M SalmonA N Akbar
Jun 10, 2009·BMC Genomics·Magdalena ChechlinskaJan Steffen
Jul 10, 2001·Journal of Glaucoma·D C Broadway, L P Chang
Dec 28, 2006·Immunological Investigations·Nasser Al-Shanti, Ziyad Aldahoudi
Jan 16, 2008·The Journal of Allergy and Clinical Immunology·Marie-Eve DarveauJamila Chakir
Nov 21, 2002·Cytokine·Danuta J HerzykPeter J Bugelski
Oct 15, 2010·Journal of Cellular Physiology·JianFei WangEdward E Tredget
Nov 26, 2010·European Journal of Immunology·Sandra Hervas-StubbsIgnacio Melero
Dec 1, 2012·Experimental Cell Research·Rui KangHerbert J Zeh Iii
May 23, 2001·International Immunology·L VivienD Mathis
May 3, 2015·The Journal of Immunology : Official Journal of the American Association of Immunologists·Saba Al HeialyJames G Martin
Oct 18, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·G LombardiA N Akbar
Jul 22, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Kenji InaClaudio Fiocchi
Jan 22, 2019·International Journal of Cell Biology·Masoumeh RostamiMajid Shahbazi
Jan 23, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·In Yong LeeJongseon Choe
Mar 26, 1999·European Journal of Immunology·D PillingM Salmon

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