Fibroblasts that overexpress 15-lipoxygenase generate bioactive and minimally modified LDL

Arteriosclerosis, Thrombosis, and Vascular Biology
F SigariP D Reaven

Abstract

Several lines of evidence suggest that the cellular enzyme 15 lipoxygenase (15-LO) may be important in promoting the oxidation of lipoproteins in vivo. In previous studies we have shown that fibroblasts transfected with 15-LO "seed" LDL with lipoperoxides such that subsequent oxidation readily generates an LDL that is taken up by macrophages through scavenger receptors. We now demonstrate that LDL incubated with 15-LO cells is "minimally modified" and has bioactive properties. Characterization of LDL incubated with 15-LO cells reveals that lipid peroxidation is modest, with low levels of TBARS generated (12.6 +/- 4.7 nmole MDA per mg protein) and small amounts of 18:2 lost as a result of oxidation (7%, compared with extensive loss [82%] with copper oxidation). The 15-LO-conditioned LDL showed mildly increased electrophoretic mobility on agarose gels, and on polyacrylamide gels it showed only mild protein degradation compared with copper-oxidized LDL. Additionally 15-LO-conditioned LDL competed very well for the LDL receptor of fibroblasts but did not compete for macrophage uptake of 125I-acetylated LDL. Importantly, compared with LDL incubated on beta-galactosidase (lac Z)-transfected control cells, LDL incubated on 15-LO cells...Continue Reading

References

Sep 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·S Ylä-HerttualaD Steinberg
Nov 1, 1991·Arteriosclerosis and Thrombosis : a Journal of Vascular Biology·G BallaG M Vercellotti
Jun 1, 1991·The Journal of Clinical Investigation·F LiaoA M Fogelman
Apr 1, 1990·The Journal of Clinical Investigation·J A BerlinerA M Fogelman
Apr 6, 1989·The New England Journal of Medicine·D SteinbergJ L Witztum
Oct 1, 1989·The Journal of Clinical Investigation·S Ylä-HerttualaD Steinberg
Mar 4, 1988·Biochimica Et Biophysica Acta·U P Steinbrecher
May 1, 1986·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·J A BerlinerA M Fogelman
Jul 1, 1995·The Journal of Clinical Investigation·V A FolcikM K Cathcart
Mar 1, 1994·Coronary Artery Disease·M NavabA M Fogelman
Apr 1, 1993·European Journal of Biochemistry·J BelknerH Kühn
Jan 1, 1996·Free Radical Biology & Medicine·J A Berliner, J W Heinecke
Aug 1, 1959·Canadian Journal of Biochemistry and Physiology·E G BLIGH, W J DYER

❮ Previous
Next ❯

Citations

Jan 19, 2002·Biochemical and Biophysical Research Communications·Kristina JostarndtJiri Neuzil
Aug 18, 2000·Free Radical Biology & Medicine·M K Cathcart, V A Folcik
Nov 24, 1999·Free Radical Biology & Medicine·R Brigelius-Flohé
Nov 1, 2001·Trends in Cardiovascular Medicine·C D Funk, T Cyrus
Feb 12, 2008·The Journal of Biological Chemistry·Richard HarkewiczYury I Miller
Jun 30, 2007·Antioxidants & Redox Signaling·Gerd Schmitz, Margot Grandl
Nov 6, 2009·Antioxidants & Redox Signaling·Irena LevitanPapasani V Subbaiah
Apr 15, 2011·Journal of Lipids·Irena Levitan, Tzu-Pin Shentu
Jul 19, 2008·Arteriosclerosis, Thrombosis, and Vascular Biology·Melissa H NagelinCatherine C Hedrick
Jun 17, 2011·Arteriosclerosis, Thrombosis, and Vascular Biology·Klaus LeyCatherine C Hedrick
Feb 5, 2000·American Journal of Respiratory Cell and Molecular Biology·D J Conrad, M Lu
Jun 8, 1999·The Journal of Clinical Investigation·T CyrusC D Funk
Apr 13, 2012·Journal of Lipid Research·Tzu Pin ShentuIrena Levitan
May 6, 2010·Clinical Chemistry and Laboratory Medicine : CCLM·Martin Hersberger
Sep 18, 2007·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Jonas Wittwer, Martin Hersberger
Jan 10, 2009·Atherosclerosis·Martin HersbergerThomas Illig
Dec 11, 2007·Atherosclerosis·Kristina Norén DanielssonLillemor Mattsson Hultén
Apr 23, 2004·Journal of Lipid Research·Catherine A ReardonGodfrey S Getz
Mar 22, 2012·Medicinal Research Reviews·Valérie CapraG Enrico Rovati
Sep 3, 1999·The Journal of Biological Chemistry·G M ChisolmM K Cathcart
Nov 13, 1998·Current Opinion in Lipidology·J L Witztum, J A Berliner
May 8, 2003·The Journal of Biological Chemistry·Melissa E HatleyCatherine C Hedrick
Dec 17, 2003·The Journal of Biological Chemistry·Kelly B ReillyCatherine C Hedrick
Mar 14, 2000·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·C PeruginiG Bellomo
Nov 26, 2018·Progress in Lipid Research·Nikhlesh K Singh, Gadiparthi N Rao
Oct 26, 2010·Progress in Lipid Research·Anca D DobrianJerry L Nadler

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

ApoE, Lipids & Cholesterol

Serum cholesterol, triglycerides, apolipoprotein B (APOB)-containing lipoproteins (very low-density lipoprotein (VLDL), immediate-density lipoprotein (IDL), and low-density lipoprotein (LDL), lipoprotein A (LPA)) and the total cholesterol/high-density lipoprotein (HDL) cholesterol ratio are all connected in diseases. Here is the latest research.