Fibrocyte migration, differentiation and apoptosis during the corneal wound healing response to injury

Experimental Eye Research
Luciana LassanceSteven E Wilson

Abstract

The aim of this study was to determine whether bone marrow-derived fibrocytes migrate into the cornea after stromal scar-producing injury and differentiate into alpha-smooth muscle actin (αSMA) + myofibroblasts. Chimeric mice expressing green fluorescent protein (GFP) bone marrow cells had fibrosis (haze)-generating irregular phototherapeutic keratectomy (PTK). Multiplex immunohistochemistry (IHC) for GFP and fibrocyte markers (CD34, CD45, and vimentin) was used to detect fibrocyte infiltration into the corneal stroma and the development of GFP+ αSMA+ myofibroblasts. IHC for activated caspase-3, GFP and CD45 was used to detect fibrocyte and other hematopoietic cells undergoing apoptosis. Moderate haze developed in PTK-treated mouse corneas at 14 days after surgery and worsened, and persisted, at 21 days after surgery. GFP+ CD34+ CD45+ fibrocytes, likely in addition to other CD34+ and/or CD45+ hematopoietic and stem/progenitor cells, infiltrated the cornea and were present in the stroma in high numbers by one day after PTK. The fibrocytes and other bone marrow-derived cells progressively decreased at four days and seven days after surgery. At four days after PTK, 5% of the GFP+ cells expressed activated caspase-3. At 14 days aft...Continue Reading

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Citations

Nov 28, 2018·Investigative Ophthalmology & Visual Science·Paramananda SaikiaSteven E Wilson
Feb 16, 2019·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·A Sue MenkoMary Ann Stepp
Feb 23, 2020·Investigative Ophthalmology & Visual Science·Rodrigo Carlos de Oliveira, Steven E Wilson
Jul 2, 2019·International Journal of Molecular Medicine·Yingzheng WengLijiang Tang
Aug 31, 2019·Investigative Ophthalmology & Visual Science·Xiaolei WangQingjun Zhou
Aug 21, 2020·Translational Vision Science & Technology·Rodrigo Carlos de OliveiraSteven E Wilson
Aug 12, 2018·Investigative Ophthalmology & Visual Science·Carla S MedeirosSteven E Wilson
Mar 19, 2019·Investigative Ophthalmology & Visual Science·Carla S MedeirosSteven E Wilson
Oct 9, 2020·Scientific Reports·Paramananda SaikiaSteven E Wilson
Sep 10, 2020·Experimental Eye Research·Rodrigo Carlos de Oliveira, Steven E Wilson
Nov 18, 2020·Translational Vision Science & Technology·Patrick M McNutt, Rajiv R Mohan
May 23, 2020·Experimental Eye Research·Gary H F YamVishal Jhanji
Oct 18, 2020·Experimental Eye Research·Steven E WilsonRodrigo Carlos de Oliveira
Jun 20, 2020·Experimental Eye Research·Steven E Wilson
Oct 4, 2020·Experimental Eye Research·Steven E Wilson
Sep 24, 2018·Experimental Eye Research·Patricia Gallego-MuñozM Carmen Martínez-García
Apr 25, 2021·Experimental Eye Research·Steven E Wilson
Jun 8, 2021·Experimental Eye Research·Lawson Ung, James Chodosh
Sep 7, 2021·Journal of Cellular and Molecular Medicine·Nina SeidelmannMatthias Fuest

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