Fibromodulin reprogrammed cells: A novel cell source for bone regeneration

Biomaterials
Chen-Shuang LiChia Soo

Abstract

Pluripotent or multipotent cell-based therapeutics are vital for skeletal reconstruction in non-healing critical-sized defects since the local endogenous progenitor cells are not often adequate to restore tissue continuity or function. However, currently available cell-based regenerative strategies are hindered by numerous obstacles including inadequate cell availability, painful and invasive cell-harvesting procedures, and tumorigenesis. Previously, we established a novel platform technology for inducing a quiescent stem cell-like stage using only a single extracellular proteoglycan, fibromodulin (FMOD), circumventing gene transduction. In this study, we further purified and significantly increased the reprogramming rate of the yield multipotent FMOD reprogrammed (FReP) cells. We also exposed the 'molecular blueprint' of FReP cell osteogenic differentiation by gene profiling. Radiographic analysis showed that implantation of FReP cells into a critical-sized SCID mouse calvarial defect, contributed to the robust osteogenic capability of FReP cells in a challenging clinically relevant traumatic scenario in vivo. The persistence, engraftment, and osteogenesis of transplanted FReP cells without tumorigenesis in vivo were confirmed...Continue Reading

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Citations

Oct 30, 2016·The American Journal of Pathology·Zhong ZhengChia Soo
Dec 6, 2018·Proteomics. Clinical Applications·Xiang-Juan LiHe-Feng Huang
Dec 5, 2017·Signal Transduction and Targeted Therapy·Zhong ZhengChia Soo
Jul 16, 2019·The Journal of Clinical Investigation·Zhong ZhengChia Soo
Feb 23, 2020·Frontiers in Pharmacology·Tanaya Walimbe, Alyssa Panitch
Feb 18, 2020·Frontiers in Pharmacology·Xiaoxiao PangZhong Zheng
Oct 5, 2017·Scientific Reports·Vardit KramMarian F Young
Jan 11, 2020·Frontiers in Pharmacology·Chenshuang LiMin Zou
Jan 3, 2018·Journal of Cranio-maxillo-facial Surgery : Official Publication of the European Association for Cranio-Maxillo-Facial Surgery·Jonathan M BekiszPaulo G Coelho
Mar 30, 2021·Regenerative Therapy·Long OuyangDaojing Qiu

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