PMID: 7544537Aug 1, 1995Paper

Fibronectin attenuates increased endothelial monolayer permeability after RGD peptide, anti-alpha 5 beta 1, or TNF-alpha exposure

The American Journal of Physiology
T M CurtisT M Saba

Abstract

Endothelial permeability can be altered by tumor necrosis factor-alpha (TNF-alpha), a cytokine released in association with inflammation-induced tissue injury. In the subendothelial matrix, fibronectin (Fn) influences endothelial cell adhesion by the interaction of integrins with RGD and non-RGD attachment sites in Fn. We compared the effect of TNF-alpha, RGD-containing peptides (GRGDSP), or antibody to alpha 5 beta 1-integrins on the protein permeability of bovine lung endothelial monolayers as assessed by transendothelial 125I-labeled albumin clearance. We also examined the influence of purified human plasma fibronectin (hFn) on this permeability response. TNF-alpha, RGD peptides, and antibodies to alpha 5 beta 1-integrins elicited a dose- and time-dependent increase in protein permeability as well as a reorganization and/or disruption of the endogenous Fn matrix. A control RGE peptide (GRGESP) as well as immunoglobulin G purified from nonimmune rabbit serum did not increase endothelial protein permeability or disrupt the endogenous fibrillar Fn pattern in the matrix. Likewise, a LDV peptide derived from the alternatively spliced type III connecting segment (IIICS) within bovine Fn (bFn) was unable to increase permeability of...Continue Reading

Citations

Jul 13, 2000·American Journal of Physiology. Lung Cellular and Molecular Physiology·R ChenT M Saba
Jan 17, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·Robert F RotundoThomas M Saba
Sep 13, 2002·American Journal of Physiology. Cell Physiology·Baochong GaoMin-Fu Tsan
Dec 24, 2005·Physiological Reviews·Dolly Mehta, Asrar B Malik
Aug 10, 2007·American Journal of Physiology. Cell Physiology·Kazuyoshi OmoriHirofumi Makino
Apr 6, 2001·American Journal of Physiology. Lung Cellular and Molecular Physiology·H ZhangA S Slutsky
Sep 13, 2007·Molecular and Cellular Biochemistry·Neetu TyagiDavid Lominadze
Dec 15, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Nicoletta GiamblancoGiovanni Marletta

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