PMID: 8601737Feb 1, 1996Paper

Fibronectin degradation in chronic wounds depends on the relative levels of elastase, alpha1-proteinase inhibitor, and alpha2-macroglobulin

The Journal of Investigative Dermatology
F Grinnell, M Zhu

Abstract

The goal of our studies was to learn about the mechanism of fibronectin degradation in chronic ulcers. We found that the appearance of fibronectin fragments in chronic ulcer wound fluid correlated with elevated levels of elastase and cleavage of the proteinase inhibitors alpha2-macroglobulin (alpha2-M) and alpha 1-proteinase inhibitor (alpha1-P1). Some wound fluid samples retained the capacity to degrade fibronectin in vitro. Degradation of fibronectin by these samples was blocked by specific inhibitors of neutrophil elastase but not by inhibitors of metalloproteinases. Addition of human neutrophil elastase to mastectomy fluid, an acute wound fluid, resulted in formation of alpha1-PI and alpha2-M complexes and cleavage products resembling those observed in chronic wound fluid. Moreover, degradation of fibronectin and processing of matrix metalloproteinase MMP-9 occurred under these conditions. Taken together, our findings suggest that elevated levels of neutrophil elastase are responsible for fibronectin degradation in the chronic wound environment.

References

Nov 1, 1992·The Journal of Investigative Dermatology·W Y ChenM J Lydon
Dec 11, 1992·The Journal of Investigative Dermatology·M S AgrenP M Mertz
Oct 1, 1991·The American Review of Respiratory Disease·J C WilliamsR D Krell
Jan 30, 1990·Biochemical and Biophysical Research Communications·S MehdiP Bey
Feb 1, 1987·Acta Ophthalmologica·E M SalonenA Vaheri
Oct 1, 1989·British Journal of Rheumatology·T E CawstonB L Hazleman
Jan 15, 1988·The Biochemical Journal·M C Vissers, C C Winterbourn
Jun 1, 1988·The Journal of Investigative Dermatology·P J JensenG S Lazarus
Jun 1, 1988·The Journal of Investigative Dermatology·J Grøndahl-HansenK Danø
Nov 15, 1980·The Biochemical Journal·G MurphyJ J Reynolds
Jul 1, 1983·Archives of Ophthalmology·T NishidaR Manabe
Aug 1, 1994·British Journal of Rheumatology·G PartschM Matucci-Cerinic
Feb 1, 1995·The Journal of Investigative Dermatology·E C BullenE W Howard
Dec 1, 1994·American Journal of Respiratory and Critical Care Medicine·G Döring
Nov 1, 1994·The Journal of Investigative Dermatology·P K Young, F Grinnell
Nov 1, 1994·The British Journal of Dermatology·M S Agren
Feb 1, 1993·Experimental Dermatology·M PalolahtiA Vaheri
Dec 1, 1993·Journal of Cellular Physiology·S DesrivièresS Ménashi
Jan 1, 1994·The Journal of Investigative Dermatology·V FalangaA Moshell
Jul 1, 1993·American Journal of Surgery·N T Bennett, G S Schultz
Jul 1, 1993·The Journal of Investigative Dermatology·A B WysockiF Grinnell
Jan 1, 1993·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·H Birkedal-HansenJ A Engler
May 1, 1993·The British Journal of Surgery·M C StaceyB S Bhogal
Jul 1, 1993·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·B BucaloV Falanga

❮ Previous
Next ❯

Citations

May 7, 1999·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·C HeF Arnold
Apr 25, 2000·Journal of Wound Care·R HoffmanM Eagle
Jul 11, 2000·British Journal of Nursing : BJN·L Russell
May 2, 2002·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Breda CullenLibby Morrison
Apr 15, 2005·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Ewen Smith, Richard Hoffman
Jun 15, 2007·The International Journal of Lower Extremity Wounds·Magnus S Agren, Maria Werthén
Dec 1, 2007·The International Journal of Lower Extremity Wounds·Dorne R YagerLaura A Gilman
Apr 4, 2008·Advances in Skin & Wound Care·Kevin Y Woo, R Gary Sibbald
Nov 12, 2009·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Andrea N MoorLisa J Gould
Dec 25, 2009·Biological Research for Nursing·Joyce StechmillerGregory Schultz
Mar 3, 2011·Journal of Biomaterials Applications·J Vincent EdwardsSteven C Goheen
Apr 15, 2011·Experimental Dermatology·Andrea HasmannEva Wehrschuetz-Sigl
Sep 20, 2011·Fibrogenesis & Tissue Repair·Wing S To, Kim S Midwood
May 1, 2012·Journal of the Chinese Medical Association : JCMA·Shih-An LiuYung-Tsung Chiu
Mar 31, 2015·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Miriam H E Blokhuis-ArkesRoland Beuk
Apr 30, 2016·Clinical Science·Sandeep DhallManuela Martins-Green
Dec 16, 2016·Plastic and Reconstructive Surgery. Global Open·Julien BaudoinWassim Raffoul
Aug 2, 2017·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Jodi C McDanielNarasimham Parinandi
Sep 3, 2020·Vascular Medicine·Jiri Kanta, Anna Zavadakova
Jul 28, 2007·Annals of the New York Academy of Sciences·Sandor SzaboXiming Xiong
Jun 7, 2005·American Journal of Medical Genetics. Part a·H L ArcherD T Pilz
Jul 17, 2008·International Wound Journal·Kevin Y Woo, R Gary Sibbald
Jul 26, 2007·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·W Y John Chen, Alan A Rogers
Sep 24, 2005·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Meinhard Wlaschek, Karin Scharffetter-Kochanek
Oct 3, 2007·Der Hautarzt; Zeitschrift für Dermatologie, Venerologie, und verwandte Gebiete·S A EmingT Krieg
Dec 8, 2010·Proteomics. Clinical Applications·James BroadbentZee Upton
Feb 27, 2013·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Nicholas S GreavesArdeshir Bayat
Aug 12, 2014·The International Journal of Lower Extremity Wounds·Valentina IzzoLuigi Uccioli
Feb 15, 2014·Advances in Wound Care·Traci A WilgusJodi C McDaniel
Feb 22, 2012·Scandinavian Journal of Infectious Diseases·Jasper N JacobsenKaren A Krogfelt
Apr 2, 2014·Advances in Wound Care·Sara M McCarty, Steven L Percival
Jul 3, 2015·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Sandeep DhallManuela Martins-Green
Jul 15, 2015·Advances in Wound Care·Eleri M JonesSteven L Percival
Dec 14, 2018·Current Dermatology Reports·Samantha EllisDanielle Tartar
Jul 14, 1998·British Journal of Nursing : BJN·R Hoffman
Mar 17, 2001·The British Journal of Dermatology·K Lundqvist, A Schmidtchen

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.