Fibronectin Facilitates Enterovirus 71 Infection by Mediating Viral Entry

Journal of Virology
Qiao-Qiao HeYing Zhu

Abstract

Fibronectin (FN) is a high-molecular-weight extracellular matrix protein that contains the RGDS motif, which is required to bind to integrins. Synthetic RGDS peptides have been reported to compete with FN to bind to the cell surface and inhibit the function of FN. Here, we identified that synthetic RGDS peptides significantly inhibit human enterovirus 71 (EV71) infection in cell cultures. In addition, mice treated with RGDS peptides and infected with EV71 had a significantly higher survival rate and a lower viral load than the control group. Because RGDS peptides affect the function of FN, we questioned whether FN may play a role in virus infection. Our study indicates that overexpression of FN enhanced EV71 infection. In contrast, knockout of FN significantly reduced viral yield and decreased the viral binding to host cells. Furthermore, EV71 entry, rather than intracellular viral replication, was blocked by FN inhibitor pretreatment. Next, we found that FN could interact with the EV71 capsid protein VP1, and further truncated-mutation assays indicated that the D2 domain of FN could interact with the N-terminal fragment of VP1. Taken together, our results demonstrate that the host factor FN binds to EV71 particles and facilita...Continue Reading

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Citations

Aug 2, 2019·Future Microbiology·Xingjian WenAnchun Cheng
Jan 12, 2020·Journal of Biomedical Science·Kyousuke Kobayashi, Satoshi Koike
Sep 5, 2019·Journal of Biomedical Science·Jing-Yi LinShin-Ru Shih
Jun 20, 2019·Journal of Biomedical Science·Collins Oduor Owino, Justin Jang Hann Chu
Aug 29, 2021·Viruses·Kyousuke Kobayashi, Satoshi Koike
Sep 30, 2021·Journal of Nanobiotechnology·Xianliang KeYuan Zhang

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