Fibronectin fibrils and growth factors stimulate anchorage-independent growth of a murine mammary carcinoma

Experimental Cell Research
R SaulnierB Elliott

Abstract

Stromal cells are important regulators of mammary carcinoma growth and metastasis. We have previously shown that a 3T3-L1 adipocyte cell line secretes hepatocyte growth factor (HGF), which stimulates proliferation of a murine mammary carcinoma (SP1) in monolayer cultures (DNA Cell Biol. 13, 1189-1897, 1994). We now examine the role of growth factors and the extracellular matrix protein fibronectin in stimulation of anchorage-independent growth of SP1 cells. Purified transforming growth factor-beta (TGF-beta) stimulated significant colony growth in soft agar cultures, whereas HGF had a lesser effect. Analysis by confocal microscopy revealed that carcinoma cell colonies contained extracellular microfibrils composed of fibronectin. Partial depletion of fibronectin from 7% FBS/agar cultures reduced the number of colonies; colony growth could be recovered by adding back exogenous fibronectin. Addition of the 70-kDa N-terminal fragment of fibronectin, which inhibits fibronectin fibril formation, reduced growth of SP1 cell colonies, but an 85-kDa fragment containing the cell binding domain did not inhibit colony growth. These findings indicate that deposition of extracellular fibronectin fibrils is necessary, but not sufficient, for a...Continue Reading

Citations

Oct 8, 1998·The Journal of Cell Biology·S BourdoulousE Ruoslahti
Nov 14, 2015·Biomolecular Concepts·Maximilian NiitLeda Raptis
Jul 13, 2002·International Journal of Cancer. Journal International Du Cancer·Christina LeBedisPnina Brodt
Mar 17, 2017·Molecular Biology of the Cell·Archana VaradarajKarthikeyan Mythreye
Dec 13, 2005·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Lachmi E JenndahlDan Baeckstrom

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