PMID: 8950199Nov 15, 1996Paper

Fibronectin fragment mediated cartilage chondrolysis. I. Suppression by anti-oxidants

Biochimica Et Biophysica Acta
G A HomandbergC Wen

Abstract

Fibronectin fragments damage cartilage in vitro by greatly enhancing metalloproteinases and suppressing proteoglycan (PG) synthesis which results in severe cartilage PG depletion. Since reactive oxygen species (ROS) have been implicated in catabolic cytokine action and preliminary data suggested that catabolic cytokines such as TNF-alpha, IL-1 alpha, IL-1 beta and IL-6 are responsible for fibronectin fragment mediated damage, selected anti-oxidants (AOs) were tested as inhibitors of cytokine. ROS and fibronectin fragment activity. Damage was measured by depletion of cartilage PG during tissue culture. The AO, N-acetylcysteine (NAC), decreased the extent of cartilage PG depletion caused by TNF-alpha and IL-1 alpha and by the ROS, hydrogen peroxide and superoxide anion, confirming that the cytokines operate through ROS and that ROS can initiate cartilage PG depletion. NAC at 0.1 and 1 mM, totally suppressed PG depletion caused by a highly potent amino-terminal 29-kDa fibronectin fragment (Fn-f) for 14 days in culture. NAC at 10 mM totally blocked Fn-f mediated PG depletion for 21 days and increased the cartilage PG content by 30% above normal levels. Glutathione (10 microM) and DMSO (1%) were also totally effective while catalase...Continue Reading

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Citations

Jun 5, 2004·Ageing Research Reviews·J Labat-Robert
Jun 13, 2013·Cellular and Molecular Life Sciences : CMLS·Josephine M J StoffelsWia Baron
Jan 3, 2009·Phytochemistry·Thanyaluck PhitakPrachya Kongtawelert
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Dec 31, 2003·Experimental Gerontology·Karo Gosselin, Corinne Abbadie

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