PMID: 8950200Nov 15, 1996Paper

Fibronectin fragment mediated cartilage chondrolysis. II. Reparative effects of anti-oxidants

Biochimica Et Biophysica Acta
G A HomandbergC Wen

Abstract

In an accompanying manuscript, it was shown that the cartilage chondrolytic activities of fibronectin fragments (Fn-f), which are mediated through catabolic cytokines such as TNF-alpha, IL-1 and IL-6, could be suppressed by anti-oxidants (AOs). The AOs neutralized reactive oxygen species (ROS) which are known to mediate catabolic cytokine action. The objective in this work was to test whether AOs would promote restoration of proteoglycan (PG) in Fn-f treated cartilage, since under normal culturing conditions, PG is not restored after removal of the Fn-f. Cartilage was first cultured with an amino-terminal 29-kDa Fn-f to cause loss of about half of the total PG and then treated with NAC (1 and 10 mM) or glutathione (10 microM) or DMSO (0.1 or 1%). Treatment with NAC and glutathione maximally caused restoration of PG within 14 days to normal or supernormal levels, while DMSO was less effective. Catalase, but not superoxide dismutase, enhanced PG content to a small but significant extent. The restoration of PG in Fn-f treated cartilage occurred throughout the full depth of the cartilage slices as shown by histochemical analysis. However, removal of the AO allowed a subsequent decrease in PG content suggesting that the AOs had not ...Continue Reading

References

Oct 1, 1992·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·C RathakrishnanM L Tiku
Nov 15, 1993·Archives of Biochemistry and Biophysics·D XieG A Homandberg

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Citations

Jun 13, 2013·Cellular and Molecular Life Sciences : CMLS·Josephine M J StoffelsWia Baron
Mar 14, 1998·Osteoarthritis and Cartilage·G A HomandbergJ M Williams
Jul 5, 2007·The American Journal of Sports Medicine·Brent P HansenRobert W Townsley
Feb 24, 2004·The American Journal of Sports Medicine·Damon H PettyJames R Andrews
Oct 18, 2001·Clinical Orthopaedics and Related Research·G A Homandberg

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