Fibronectin induces matrix metalloproteinase-9 (MMP-9) in human laryngeal carcinoma cells by involving multiple signaling pathways

Biochimie
Triparna SenAmitava Chatterjee

Abstract

Cell adhesion to extracellular matrix initiates intracellular signaling cascade regulated by integrin family of receptors. Evidences show that cultured cells in presence of extracellular matrix adhesion molecule Fibronectin (FN) stimulates secretion of matrix metalloproteinases (MMPs), facilitating cancer cell invasion. Amongst all MMPs, MMP-9 is often reported to play crucial role in tumor cell growth and metastasis. The present study aims at examining the effects of FN on MMP-9 in laryngeal carcinoma cell line, HEp-2, and understand the molecular mechanism(s) involved. The study reports that FN induces the gelatinolytic activity, mRNA and protein expression of MMP-9 in HEp-2 cells. This effect appears to be mediated mainly by integrin receptor α5β1, since, the blockade of α5 abrogated the FN-mediated stimulatory response on MMP-9. siRNA and inhibitor studies suggested involvement of Focal adhesion kinase (FAK), Phosphatidyl-inositol-3-kinase (PI-3K), Extracellular regulated kinase (ERK) and nuclear factor-kappaB (NFκB) in FN-mediated MMP-9 induction. Immunocytochemical analysis demonstrated the nuclear localization of ERK, PI-3K and NFκB; immunoblot showed enhanced expression of p-FAK, p-PI-3K, p-ERK and nuclear-NF-κB and ind...Continue Reading

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Citations

Sep 8, 2010·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Gargi MaityAmitava Chatterjee
May 27, 2014·Cancer Microenvironment : Official Journal of the International Cancer Microenvironment Society·Sekhar PalAmitava Chatterjee
Aug 13, 2011·Journal of Cancer Research and Clinical Oncology·Bin-Quan WangPing-Chang Yang
Apr 7, 2011·International Journal of Cancer. Journal International Du Cancer·Hongyu GuanMengfeng Li
May 10, 2012·Cell Adhesion & Migration·Grant A Howe, Christina L Addison
Jan 23, 2019·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Carina CohenFlávio Faloppa
Apr 13, 2019·Journal of Cellular Biochemistry·Yuzhen ZhouYan Huang
Aug 6, 2020·International Journal of Molecular Sciences·Vidhi MalikRenu Wadhwa
Jan 8, 2021·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Andrew Leask

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