Fibronectin inhibits morphological changes in cultures of vascular smooth muscle cells

Journal of Cellular Physiology
M J BrennanK E Fritz


In culture, vascular smooth muscle cells proliferate until they form a confluent sheet of cells. At that time the morphology of the culture becomes altered and the cells form multilayered regions that eventually develop into nodular aggregations. We now demonstrate that the transition from monolayer culture to nodular culture is influenced by the presence of components in conditioned media. The development of nodules is enhanced by conditioned medium made from nodular cultures but is either inhibited or unaffected by monolayer culture-conditioned medium. Examination of the two types of conditioned media using NaDodSO4- polyacrylamide gels reveals many similarities and one major difference. Nodular-conditioned medium contains a prominent 42 kilodalton polypeptide which is not present in monolayer-conditioned medium. Further, we demonstrate that although both nodular and monolayer cultures produce fibronectin the transition to nodular culture does not occur in the presence of exogeneously added plasma fibronectin.


Sep 1, 1979·Analytical Biochemistry·P J McCormickA J Millis
Jan 1, 1979·Physiological Reviews·J H Chamley-CampbellR Ross
Jun 1, 1977·Atherosclerosis·J M AgustynJ Jarmolych
Jan 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·P A JonesW Gevers
Jun 20, 1978·Annals of the New York Academy of Sciences·L B ChenP H Gallimore
Jul 1, 1979·Experimental Cell Research·J P PennypackerR M Pratt
Feb 1, 1979·The Journal of Cell Biology·K M Yamada, D W Kennedy
Sep 1, 1979·Experimental Cell Research·T R PodleskiK M Yamada
Jan 1, 1977·Advances in Experimental Medicine and Biology·J AugustynJ Jarmolych
Jan 1, 1977·Advances in Experimental Medicine and Biology·A S DaoudT P Mawhinney
May 17, 1976·Biochemical and Biophysical Research Communications·M J BarnesC I Levene
Jul 15, 1977·International Journal of Cancer. Journal International Du Cancer·E Engvall, E Ruoslahti
Oct 1, 1977·Journal of Cellular Physiology·A J MillisB Field
Mar 1, 1968·Journal of Atherosclerosis Research·R W Issler
May 1, 1981·The Journal of Cell Biology·J H Chamley-CampbellR Ross
Apr 1, 1980·Journal of Cellular Physiology·S Chandrasekhar, A J Millis


Aug 3, 1999·Journal of Cellular Physiology·C L Moulson, A J Millis
Feb 13, 2001·Journal of Cellular Physiology·A J MillisC L Moulson
Mar 1, 1994·Matrix Biology : Journal of the International Society for Matrix Biology·J L RuckmanJ M Davidson
Jun 12, 2003·Experimental Cell Research·Kimi C Nishikawa, Albert J T Millis
May 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·A J MillisM J Brennan
May 26, 2004·Biochemical and Biophysical Research Communications·Yoshikazu MiwaToshiyuki Sasaguri
May 1, 1987·Journal of Cellular Physiology·J SottileA J Millis
Feb 1, 1988·Journal of Cellular Physiology·A J Millis, M Hoyle
Jul 1, 1986·Cytometry·P A DennisC F Moyer

Related Concepts

Cell Adhesion
Cell Density
Muscle, Smooth, Vascular

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.