Fibronectin modulates the morphology of osteoblast-like cells (MG-63) on nano-grooved substrates.

Journal of Materials Science. Materials in Medicine
Wei-Bor TsaiHsuan-Liang Liu

Abstract

Cell interactions with biomaterials are affected by surface topographic and chemical cues. Although it is well-known that nanometrical grooves/ridges structure modulates cellular spreading, elongation, and alignment, the combinational influence of surface topographic and chemical cues is not well studied. In this study, nano-textured silicon substrata with parallel ridges of 90, 250, or 500 nm wide, separated by grooves with equal width, were fabricated by electron beam lithography and dry etching techniques. Osteoblast-like cells, MG-63, were cultured on the patterned substrata with or without pre-adsorption of fibronectin. The cell morphology was imaged by scanning electron microscopy, and analyzed by image software. We found that FN coating initially modulated cellular spreading, length, and orientation on all types of grooved surfaces. However, after 24 h of culture, the cell morphology was not affected by FN coating on the 250-nm and 500-nm surfaces, while FN decreased cell alignment on the 90-nm surfaces. Our results suggest that surface chemical cues influence the initial cell-substratum contact, while the long-term cellular morphology is dictated by surface topographic cues.

References

Jul 1, 1979·Experimental Cell Research·P T Ohara, R C Buck
Jun 1, 1986·Scientific American·R O Hynes
Jan 1, 1987·Annual Review of Cell Biology·G R Martin, R Timpl
Jan 1, 1987·Journal of Cell Science. Supplement·J A DowC D Wilkinson
Nov 1, 1986·Experimental Cell Research·D M Brunette
Jan 1, 1986·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·K J FriedmanM Nash
May 1, 1981·Journal of Cellular Physiology·D GospodarowiczN Savion
Jan 1, 1995·Parasitology Research·K TerasakiK Kawashima
Jan 1, 1995·Cell Motility and the Cytoskeleton·B Wójciak-StothardC D Wilkinson
Mar 15, 1996·Experimental Cell Research·B Wójciak-StothardC Wilkinson
Dec 22, 1998·Journal of Biomaterials Science. Polymer Edition·A S Curtis, C D Wilkinson
Mar 26, 1999·Journal of Biomaterials Science. Polymer Edition·W B Tsai, T A Horbett
Jun 24, 1999·Journal of Biomedical Materials Research·X F WalboomersJ A Jansen
Jul 6, 2000·Journal of Biomedical Materials Research·X F WalboomersJ A Jansen
Mar 7, 2001·Biology of the Cell·V Petit, J P Thiery
Nov 21, 2001·Nature Reviews. Molecular Cell Biology·B GeigerK M Yamada
Mar 26, 2003·Experimental Cell Research·Matthew J DalbyAdam S G Curtis
Apr 15, 2003·Journal of Cell Science·Ana I TeixeiraPaul F Nealey
Oct 8, 2004·Journal of Biomedical Materials Research. Part a·Ana I TeixeiraChristopher J Murphy
Jun 20, 2008·Journal of Biomedical Materials Research. Part a·Jung-Yen YangWei-Bor Tsai

❮ Previous
Next ❯

Citations

Dec 17, 2009·Journal of Materials Science. Materials in Medicine·Patricia M DavidsonKarine Anselme
Aug 21, 2012·Journal of Materials Science. Materials in Medicine·Peng-Yuan WangWei-Bor Tsai
Apr 26, 2013·Journal of Materials Science. Materials in Medicine·S GrigorescuO Gallet
Aug 16, 2011·Acta Biomaterialia·Wei-Bor TsaiMeng-Jiy Wang
Jun 15, 2011·Current Opinion in Chemical Biology·Luisa Mayorga Szott, Thomas A Horbett
Apr 6, 2013·Journal of Biomedical Materials Research. Part a·Subhash SistaGopal Pande
Dec 18, 2013·Journal of Biomedical Materials Research. Part a·Yan LiXiaoping Yang
Feb 12, 2010·Biotechnology and Bioengineering·Peng-Yuan WangWei-Bor Tsai
Jun 4, 2014·Biotechnology and Bioengineering·Petra B LückerAlison P McGuigan
Apr 22, 2017·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Noura AlomLisa J White
Jan 2, 2014·Journal of Biomaterials Applications·Wei-Bor TsaiMeng-Jiy Wang
Aug 23, 2011·Lab on a Chip·William F HeinzJan H Hoh
Mar 31, 2015·Nanomedicine·Omid MashinchianMorteza Mahmoudi
Feb 23, 2020·Journal of Functional Biomaterials·Marcella SalvatoreLuigi Ambrosio

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.