PMID: 9557937Apr 29, 1998Paper

Fibronectin production by cultured human lung fibroblasts in three-dimensional collagen gel culture

In Vitro Cellular & Developmental Biology. Animal
Y AdachiS I Rennard

Abstract

In vivo, fibroblasts are distributed in a three-dimensional (3-D) connective tissue matrix. Fibronectin is a major product of fibroblasts in routine cell culture and is thought to regulate many aspects of fibroblast biology. In this context, we sought to determine if the interaction of fibroblasts with a 3-D matrix might affect fibronectin production. To examine this hypothesis, fibronectin production by fibroblasts cultured in a 3-D collagen gel or on plastic dishes was measured by ELISA. Fibroblasts in 3-D gel culture produced more fibronectin than those in monolayer culture. Fibroblasts in 3-D culture produced increasing amounts of fibronectin when the collagen concentration of the gel was increased. The 3-D nature of the matrix appeared to be crucial because plating the fibroblasts on the surface of a plastic dish underneath a collagen gel was not different from plating them on a plastic dish in the absence of collagen. In addition to increased fibronectin production, the distribution of the fibronectin produced in 3-D culture was different from that of monolayer culture. In monolayer culture, more than half of the fibronectin was released into the culture medium. In 3-D culture, however, approximately two-thirds remained i...Continue Reading

References

Mar 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·E BellC Merrill
Jul 15, 1977·International Journal of Cancer. Journal International Du Cancer·E Engvall, E Ruoslahti
Nov 7, 1977·Biochemical and Biophysical Research Communications·B J BaumR G Crystal
Aug 1, 1992·American Journal of Respiratory Cell and Molecular Biology·D J RombergerS I Rennard
Jan 1, 1992·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·J D BeckmannS I Rennard
Jul 1, 1989·American Journal of Respiratory Cell and Molecular Biology·S ShojiS I Rennard
Nov 1, 1986·Experimental Cell Research·P GilleryJ P Borel
Nov 1, 1982·The Journal of Cell Biology·R O Hynes, K M Yamada
Mar 1, 1980·Analytical Biochemistry·C Labarca, K Paigen
Oct 1, 1984·Collagen and Related Research·B NusgensE Bell
Jun 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·J K CzopK F Austen
May 1, 1980·Analytical Biochemistry·S I RennardP G Robey
Jan 1, 1982·Methods in Enzymology·S C Strom, G Michalopoulos
Jan 1, 1982·The Journal of Clinical Investigation·S I Rennard, R G Crystal
Feb 1, 1994·The Journal of Cell Biology·F Grinnell
Jul 1, 1996·In Vitro Cellular & Developmental Biology. Animal·T MioS I Rennard

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Citations

Oct 29, 2003·Tissue & Cell·S CarnevaliS I Rennard
Sep 11, 2012·Integrative Biology : Quantitative Biosciences From Nano to Macro·Wesley R LegantViola Vogel
Mar 17, 2007·American Journal of Respiratory Cell and Molecular Biology·Koichiro KamioStephen I Rennard
Nov 20, 2003·Respiratory Research·Ulrika ZagaiC Magnus Sköld
Nov 1, 2007·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Kenneth R WattersonSarah Spiegel
Aug 15, 2008·Respiratory Medicine·Tadashi KohyamaTakahide Nagase
Sep 8, 2009·The Journal of Allergy and Clinical Immunology·Harsha H KariyawasamDouglas S Robinson
Aug 12, 2006·Burns : Journal of the International Society for Burn Injuries·Yucheng DaiGuohui Li
Oct 30, 2015·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Jegan AthinarayananAli A Alshatwi
May 6, 2003·Osteoporosis International : a Journal Established As Result of Cooperation Between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·Xiangde LiuStephen I Rennard
Nov 8, 2014·Biomaterials Science·Megan E SmithmyerApril M Kloxin
Apr 10, 2008·International Journal of Surgical Pathology·Suzana Orsini Machado de SousaVera Cavalcanti de Araújo
Mar 11, 2004·Clinical and Experimental Immunology·U ZagaiJ Lundahl
Apr 17, 2004·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Mark A Carlson, Michael T Longaker
Apr 27, 2000·American Journal of Physiology. Lung Cellular and Molecular Physiology·X D LiuS I Rennard
Jun 16, 2018·American Journal of Physiology. Heart and Circulatory Physiology·JoAnn Trial, Katarzyna A Cieslik
Dec 24, 2020·Cancer Immunology Research·Jessica WagnerStephen Gottschalk
Apr 24, 2021·Signal Transduction and Targeted Therapy·Jiacheng HuangYiting Qiao

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