Fibronectin protein expression in renal cell carcinoma in correlation with clinical stage of tumour

Biomarker Research
Sandeep KondisettyGinil Kumar Pooleri

Abstract

Carcinogenesis is a multistep process which involves interplay between the tumour cells and the matrix proteins. This occurs by adherence between the tumour cells and proteins in the extracellular matrix. VHL mutation affects through the hypoxia inducible factor (HIF) and causes changes in various tissue proteins like VEGF, PDGF, TGF, Fibronectin and others. As not much literature is available, we aim to quantify the changes of fibronectin protein in renal cell carcinoma (RCC) tissue. This Prospective unbalanced case control study was conducted over a period of 18 months from April 2016 to September 2017. The patients undergoing nephrectomy for the diagnosis of RCC were included in the study after obtaining written informed consent. Patients were excluded from study, if normal renal tissue could not be identified in the resected kidney and if the artery clamp time to retrieval of tissue was more than 30 min. Fibronectin protein is estimated in the tumour tissue by gel electrophoresis and western blotting which is compared with that of normal kidney tissue of the same kidney. Results have been expressed as absolute values with standard deviation and relative expression (RE). Of the 21 patients analysed 15 showed an increase in f...Continue Reading

References

Oct 1, 1982·The American Journal of Surgical Pathology·S A FuhrmanC Limas
Jan 1, 1996·World Journal of Urology·C B WilsonR M Nakamura
Jun 20, 2003·Lancet·Russell R LonserEdward H Oldfield
Aug 6, 2008·Der Urologe. Ausg. A·A J SchraderR Hofmann
Sep 24, 2010·BMC Cancer·Sandra WaalkesJürgen Serth
Aug 28, 2012·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Karen Cristina Barbosa ChavesMaria Helena Bellini
May 9, 2015·Familial Cancer·Narendranath VikkathAshok B Pillai
May 10, 2017·Indian Journal of Cancer·G P AbrahamE Manuel

❮ Previous
Next ❯

Citations

Jun 12, 2019·International Journal of Molecular Sciences·Yen-Chuan OuChun-Jung Chen
Jul 1, 2020·Cancers·Sabina SanegreTomás Álvaro Naranjo

❮ Previous
Next ❯

Methods Mentioned

BETA
electrophoresis

Software Mentioned

microsoft excel
ImageJ
excel
SPSS

Related Concepts

Related Feeds

Arterial-Venous in Development & Disease

Arterial-venous development may play a crucial role in cardiovascular diseases. Here is the latest research.