PMID: 7545439Jul 1, 1995Paper

Fibronectin receptors in preimplantation development: cloning, expression, and localization of the alpha 5 and beta 1 integrin subunits in bovine trophoblast

Biology of Reproduction
L A MacLaren, A G Wildeman

Abstract

The fibronectin receptor, alpha 5 beta 1, may be involved in many aspects of early development, including migration of endodermal and mesodermal cells during formation of the placenta, trophoblastic outgrowth in culture, and development of an invasive phenotype by fetal cytotrophoblasts. In contrast to the human blastocyst, the bovine blastocyst elongates in the uterine lumen for several days until it begins attachment, and the fetal trophoblast limits its invasion to the maternal epithelium. Fibronectin receptor expression was characterized in bovine embryos before and after their attachment to the uterus. Initially, the polymerase chain reaction (PCR) was conducted with degenerate oligonucleotide primers to isolate bovine cDNAs for the alpha 5 and beta 1 subunits. Bovine-specific primers were then constructed to assay for alpha 5 and beta 1 mRNA expression in embryo RNA during the morula through the attachment stages using reverse-transcriptase PCR. Northern blot analysis was used to quantify mRNA levels from Days 15 to 21. Integrin and fibronectin protein expression was assessed by immunohistochemical examination of embryo sections. Both alpha 5 and beta 1 subunit mRNAs were expressed throughout the stages examined. Expressi...Continue Reading

Citations

Sep 6, 2000·Veterinary Immunology and Immunopathology·Y QianA J Ainsworth
Jun 29, 1999·Journal of Periodontal Research·S IvanovskiA S Narayanan
Nov 19, 2013·PloS One·Noelia LosinoAlejandra Guberman
May 8, 2014·Reproduction : the Official Journal of the Society for the Study of Fertility·Rulan BaiToshihiro Sakurai
Jun 30, 2000·Seminars in Cell & Developmental Biology·J A Bowen, R C Burghardt
May 9, 2016·Theriogenology·J-D HaegerC Pfarrer
Mar 8, 2005·The Journal of Reproduction and Development·Masashi TakahashiAkira Okano
May 22, 2002·Biology of Reproduction·Wanyi Xiang, Leslie A MacLaren
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Jun 2, 2005·In Vitro Cellular & Developmental Biology. Animal·Coreyayne Singleton, Alfred R Menino
Dec 17, 2014·Periodontology 2000·Higinio ArzateGabriela Mercado-Celis
Jun 23, 2012·Biology of Reproduction·Toshihiro SakuraiKazuhiko Imakawa
Apr 23, 2002·Biology of Reproduction·Doris M MacIntyreLeslie A MacLaren
Apr 20, 2002·In Vitro Cellular & Developmental Biology. Animal·Kelly R Schilperoort-Haun, Alfred R Menino
Jun 10, 2004·Acta histochemica·Sevinc InanSevil Sayhan

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