Fibronectin splicing variants in human intervertebral disc and association with disc degeneration

Spine
D Greg AndersonYejia Zhang

Abstract

In this study, normal intervertebral disc (IVD) tissues and degenerative human IVD tissues were compared for presence of fibronectin (FN) mRNA splice variants and for FN fragments (FN-f). To further understand FN RNA splice forms and protein fragments in disc degeneration. FN splice variants play important roles in regulating cell-matrix and matrix-matrix interactions in skeletogenesis and skeletal function in limbs and other sites. However, presence and possible roles of FN splice variants and fragments in human IVD have not been determined. Normal infant and adult IVD tissues were obtained from organ donors, and degenerative human IVD tissues were obtained from patients undergoing spinal surgeries. FN splice patterns were assessed by reverse transcriptase polymerase chain reaction. Relative expression levels were semiquantified by densitometry. FN and its fragments were studied by Western blot analysis. Both the EDB and EDB splice variants were present in normal and degenerative IVD tissues. The EDB to EDB ratio was highest in moderately degenerative tissue. The EDA domain was only expressed in infant but not adult tissue. Variable-region (V) splice forms were present in all tissues studied. A splice form with the entire V-re...Continue Reading

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Citations

Apr 18, 2013·European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·Sarit Sara SivanSally Roberts
Mar 30, 2013·The Spine Journal : Official Journal of the North American Spine Society·Jillian E MayerAndrew C Hecht
Nov 21, 2014·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Carla R ScanzelloYejia Zhang
Mar 19, 2013·The American Journal of Pathology·André Nicki Tiaden, Peter James Richards
Sep 2, 2017·American Journal of Physical Medicine & Rehabilitation·Zuozhen TianYejia Zhang
Sep 23, 2020·Advanced Drug Delivery Reviews·Jennifer Patten, Karin Wang

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