Fibronectin synthesis, but not α-smooth muscle expression, is regulated by periostin in gingival healing through FAK/JNK signaling

Scientific Reports
Shawna S KimDouglas W Hamilton

Abstract

During skin healing, periostin facilitates myofibroblast differentiation through a β1 integrin/FAK dependent mechanism and continued expression is associated with scarring. In contrast to skin, gingival tissue does not typically scar upon injury, but the role of periostin in gingival healing has never been investigated. Using a rat gingivectomy model, we show that the gingival architecture is re-established within 14 days of wounding. Periostin mRNA levels peak at day 7 post-wounding, with persistence of periostin protein in the connective tissue through day 14. Collagen type I and lysyl oxidase mRNA levels peak at day 7 post wounding, which corresponded with the peak of fibroblast proliferation. Although α-smooth muscle actin mRNA levels increased 200-fold in the tissue, no myofibroblasts were detected in the regenerating tissue. In vitro, human gingival fibroblast adhesion on periostin, but not collagen, was inhibited by blocking β1 integrins. Fibroblasts cultured on periostin exhibited similar rates of proliferation and myofibroblast differentiation to cells cultured on collagen only. However, human gingival fibroblasts cultured in the presence of periostin exhibited significantly increased fibronectin and collagen mRNA leve...Continue Reading

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Citations

Feb 9, 2020·International Journal of Molecular Sciences·Georgia NikoloudakiDouglas W Hamilton
Apr 9, 2020·American Journal of Physiology. Cell Physiology·Georgia NikoloudakiDouglas W Hamilton
Jan 16, 2020·Cells·Katarzyna Aleksandra KujawaKatarzyna Marta Lisowska
May 10, 2021·Journal of the Formosan Medical Association = Taiwan Yi Zhi·Jyun-Yang SuMing-Yung Chou
Jun 18, 2021·Biochimica Et Biophysica Acta. Reviews on Cancer·Weifan ZhangZheng Wang

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Methods Mentioned

BETA
PCR
ELISA
Assay
Protein assay
electrophoresis

Software Mentioned

AxioVision Relative
ImageJ
Ensembl
Graphpad
ZenPro
Leica Application Suite

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