Fibronectin synthesis in tubular epithelial cells: up-regulation of the EDA splice variant by transforming growth factor beta

Kidney International
C ViedtG M Hänsch

Abstract

The influence of transforming growth factor beta 1 (TGF-beta 1) and of dexamethasone on fibronectin (FN) synthesis of human renal tubular epithelial cells in culture (TEC) was studied. Cocultivation with TGF-beta 1 increased the steady state level of FN RNA within 24 to 48 hours. By PCR and Northern blotting it was found that the EDA splice variant of FN was preferentially up-regulated. To quantitate FN protein synthesis, cells were cultivated in the presence of [35S]-methionine and FN was isolated from the cell supernatants, and the cell lysates by adsorption to gelatin-sepharose. In TGF-beta 1 treated cells, a small increase of FN in the cell supernatants was seen (1.7-fold), and a more prominent increase in the cell lysates (4.5-fold). The FN content of the extracellular matrix was also increased in TGF-beta 1 treated cells. Most of the de novo synthesized FN was identified as the EDA-variant of FN. As a further stimulus, dexamethasone was used. Again, an increase of FN-specific mRNA was seen as well as an increased FN protein synthesis. The ratio between FN and EDA-FN, however, was not altered when compared to untreated cells. Thus, an increase in EDA-FN synthesis is obviously stimulus dependent.

References

Jul 15, 1977·International Journal of Cancer. Journal International Du Cancer·E Engvall, E Ruoslahti
Dec 1, 1992·The Journal of Cell Biology·M B Sporn, A B Roberts
Mar 1, 1992·Kidney International·A B RobertsM B Sporn
Apr 1, 1992·Journal of the American Society of Nephrology : JASN·J I ScheinmanO Brown
Jul 1, 1992·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·K A Nath
Mar 1, 1991·Kidney International·G Wolf, E G Neilson
Mar 1, 1991·Kidney International·G S KuncioT Haverty
Aug 1, 1989·The Journal of Cell Biology·C Ffrench-ConstantR O Hynes
Feb 1, 1985·The Journal of Urology·A L TrifillisB F Trump
Jun 23, 1988·The New England Journal of Medicine·S KlahrI Ichikawa
Aug 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·J W TamkunR O Hynes
Jun 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·A R KornblihttF E Baralle
Apr 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·G M Church, W Gilbert
Jan 1, 1983·Annual Review of Biochemistry·K M Yamada
Apr 1, 1994·Kidney International·W W TangC B Wilson
Oct 1, 1993·Kidney International·A K SharmaA F Michael
Sep 1, 1993·Molecular and Cellular Biology·G S Huh, R O Hynes

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Citations

May 20, 1998·Clinical and Experimental Pharmacology & Physiology·M D Basson
Apr 17, 2004·Kidney International·Simone de HaijCees van Kooten
Sep 1, 1996·Kidney International·C J BurtonK P Harris
Nov 14, 1997·The Journal of Laboratory and Clinical Medicine·J A BruijnE de Heer
Jun 13, 2015·The Journal of Investigative Dermatology·Rhiannon M KelshRichard A F Clark
Jan 15, 2016·Journal of the American Society of Nephrology : JASN·Megan Stevens, Sebastian Oltean
Feb 17, 2001·The Journal of Pathology·A Van VlietJ A Bruijn
Jun 26, 2014·Journal of Cellular Physiology·Mysore Keshavmurthy PhanishMark Edward Carl Dockrell
Oct 12, 2000·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·C ViedtJ Kreuzer
Dec 9, 1998·The American Journal of Physiology·D P BasileM R Hammerman
Mar 1, 1997·The Journal of International Medical Research·Y TaniguchiM Yamakido
Jul 14, 2017·American Journal of Physiology. Renal Physiology·Fahmy A MamuyaHua A Jenny Lu
May 16, 2001·American Journal of Physiology. Renal Physiology·A ZukK S Matlin
May 20, 2020·Frontiers in Oncology·Georgios EfthymiouEllen Van Obberghen-Schilling
Jun 19, 2001·American Journal of Physiology. Heart and Circulatory Physiology·A BürgerH G Zimmer
May 23, 1998·Experimental Cell Research·L BorsiL Zardi
Feb 17, 2000·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·T ShojiY Tsubakihara

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