Fibrous proteins: At the crossroads of genetic engineering and biotechnological applications

Biotechnology and Bioengineering
Sezin YigitDavid L Kaplan

Abstract

Fibrous proteins, such as silk, elastin and collagen are finding broad impact in biomaterial systems for a range of biomedical and industrial applications. Some of the key advantages of biosynthetic fibrous proteins compared to synthetic polymers include the tailorability of sequence, protein size, degradation pattern, and mechanical properties. Recombinant DNA production and precise control over genetic sequence of these proteins allows expansion and fine tuning of material properties to meet the needs for specific applications. We review current approaches in the design, cloning, and expression of fibrous proteins, with a focus on strategies utilized to meet the challenges of repetitive fibrous protein production. We discuss recent advances in understanding the fundamental basis of structure-function relationships and the designs that foster fibrous protein self-assembly towards predictable architectures and properties for a range of applications. We highlight the potential of functionalization through genetic engineering to design fibrous protein systems for biotechnological and biomedical applications. Biotechnol. Bioeng. 2015;9999: 1-17. (c) 2015 Wiley Periodicals, Inc.

References

Nov 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·H LehrachP Doty
Dec 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·M E SobelI Pastan
Sep 1, 1977·Journal of Molecular Biology·M J Sternberg, J M Thornton
Nov 1, 1990·Trends in Biotechnology·J Cappello
Jan 1, 1992·Progress in Biophysics and Molecular Biology·D W Urry
May 1, 1991·Journal of Vascular Surgery·A NicolD W Urry
Sep 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·M Xu, R V Lewis
Mar 1, 1986·Calcified Tissue International·C G BellowsM E Antosz
Jan 1, 1971·Advances in Protein Chemistry·W Traub, K A Piez
Dec 18, 1974·Biochimica Et Biophysica Acta·D W UrryM Jacobs
Dec 21, 1973·Nature·W R GrayJ A Foster
Dec 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·C M LapièreL D Kohn
Mar 17, 1995·Biochemical and Biophysical Research Communications·N MinouraY Imai
Oct 1, 1993·Current Opinion in Cell Biology·R Mayne, R G Brewton
Sep 15, 1995·Structure·J BellaH M Berman
Jun 1, 1996·Protein Expression and Purification·R V LewisM J Fournier
Jan 1, 1997·Applied Microbiology and Biotechnology·S R Fahnestock, S L Irwin
Jan 1, 1997·Applied Microbiology and Biotechnology·S R Fahnestock, L A Bedzyk
Mar 6, 1998·Applied Microbiology and Biotechnology·S ArcidiaconoH Bayley
Mar 21, 1998·Matrix Biology : Journal of the International Society for Matrix Biology·M NokelainenK I Kivirikko
Sep 19, 1998·Journal of Controlled Release : Official Journal of the Controlled Release Society·J CappelloE R Stedronsky
Apr 20, 1999·Nature Biotechnology·D C JohnN J Bulleid
Oct 6, 1999·Current Opinion in Biotechnology·F Baneyx
Mar 22, 2000·Proceedings of the National Academy of Sciences of the United States of America·K BeckB Brodsky
Aug 30, 2000·Biochemical Society Transactions·J MyllyharjuK I Kivirikko
Nov 15, 2000·Journal of Biomedical Materials Research·S SofiaD L Kaplan
Jun 1, 2001·Nature Biotechnology·J SchellerU Conrad

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Citations

Aug 16, 2016·International Journal of Molecular Sciences·Marie-Laurence TremblayJan K Rainey
Sep 21, 2016·Journal of Functional Biomaterials·Michael FlorenAntonella Motta
Dec 17, 2016·Scientific Reports·Mário BarrocaTony Collins
Dec 13, 2016·Journal of Tissue Engineering and Regenerative Medicine·Nina DinjaskiDavid L Kaplan
Nov 14, 2017·Tissue Engineering. Part a·Arnout MieremetAbdoelwaheb El Ghalbzouri
Apr 3, 2019·Advanced Healthcare Materials·Yao WangJin Kim Montclare
Mar 12, 2020·Annual Review of Biomedical Engineering·Jung Woo LeemYoung L Kim
Aug 5, 2020·Scientific Reports·Dalia Jane SaldanhaNoémie-Manuelle Dorval Courchesne
Apr 2, 2020·Biointerphases·Markian S BahniukLarry D Unsworth

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