PMID: 6411826Sep 1, 1983Paper

Fibrous proteins of bovine hoof

The Journal of Investigative Dermatology
H P Baden, J Kubilus

Abstract

The matrix region of calf hoof was identified as the living precursor layer of the hardened hoof plate. Fibrous protein was isolated from the matrix with citrate buffer, pH. 2.65, while Tris buffer, pH. 9.5, with 8 M urea and a reducing agent was required to dissolve the cornified hoof. The purified matrix protein had a sodium dodecyl sulfate-polyacrylamide gel electrophoretic pattern similar to hoof plate and different from bovine epidermis. When the s-carboxymethyl derivatives of matrix, hoof plate, and hair proteins was compared by urea-polyacrylamide gel electrophoresis, they were identical, and different from that of epidermal protein. The matrix protein reacted with an antibody to hair fibrous protein. Cultured matrix keratinocytes appeared to be identical to cultured epidermal cells, pointing to the importance of the dermis in epidermal cell differentiation.

References

Jun 19, 1979·Biochimica Et Biophysica Acta·J KubilusH P Baden
Dec 11, 1979·Biochemistry·P M Steinert, W W Idler
May 30, 1978·Biochemical and Biophysical Research Communications·W W de JongL H Cohen
Aug 1, 1978·The Journal of Investigative Dermatology·H P Baden, L D Lee
Nov 18, 1975·Biochimica Et Biophysica Acta·L D LeeH P Baden
Oct 1, 1976·The Journal of Investigative Dermatology·L D LeeB F Fleming
Nov 1, 1976·The Journal of Investigative Dermatology·H P BadenJ Kubilus
Jul 1, 1975·The Biochemical Journal·P M Steinert
Jul 1, 1973·American Journal of Physical Anthropology·D Hrdy, H P Baden
Jan 1, 1969·The Journal of Investigative Dermatology·Y ShechterV D Newcomer
Apr 29, 1971·Biochimica Et Biophysica Acta·P M Steinert, G E Rogers
Sep 1, 1980·The Journal of Investigative Dermatology·R C Marshall
Jun 1, 1980·The Biochemical Journal·P M SteinertM L Wantz
Oct 1, 1980·The Journal of Investigative Dermatology·H P BadenJ Kubilus
Nov 1, 1980·The Journal of Investigative Dermatology·H P BadenT S Argyris

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Citations

Jul 13, 2004·Journal of Industrial Microbiology & Biotechnology·Esabi B Kurbanoglu, Namudar I Kurbanoglu
Apr 20, 2004·Australian Veterinary Journal·N G Gregory
Feb 12, 2005·Drug Development and Industrial Pharmacy·Daniela MontiF Mailland
Dec 16, 2006·Current Microbiology·E Vasileva-TonkovaA Gushterova
Jul 1, 1995·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·D O'Toole, M F Raisbeck
Aug 6, 2003·FEMS Microbiology Letters·Esabi Basaran Kurbanoglu, Namudar Izzet Kurbanoglu
Sep 14, 2002·Bioresource Technology·Esabi Basaran Kurbanoglu, Omer Faruk Algur
Dec 10, 1986·Biochimica Et Biophysica Acta·J C KvedarH P Baden

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