Fibulin-2 binds to the short arms of laminin-5 and laminin-1 via conserved amino acid sequences.

The Journal of Biological Chemistry
A UtaniY Yamada

Abstract

Epithelial cell-specific laminin-5, consisting of three chains, alpha3, beta3, and gamma2, is a component of the anchoring filament that traverses the lamina lucida beneath the hemidesmosomes of epidermal cells and functions to link these cells to the basement membrane. We have studied the molecular interaction between laminin-5 and extracellular matrix proteins using recombinant proteins and synthetic peptides. Affinity chromatography assays with recombinant fragments of the laminin gamma2 short arm identified a 195-kDa binding protein in the conditioned media from the mouse epidermal cell line Pam 212 and from primary dermal fibroblasts. This molecule was identified by Western blotting as fibulin-2, a recently identified extracellular matrix protein. Using deletion mutants and various synthetic peptides in competition assays, the 9-amino acid sequence SADFSVHKI (residues 199-207) in domain IV of the gamma2 chain was defined as a critical site for fibulin-2 binding. An anti-gamma2 antibody co-immunoprecipitated fibulin-2 from the conditioned media, further confirming the interaction of fibulin-2 with laminin-5. Fibulin-2 was also found to interact with laminin-1 (alpha1beta1gamma1) through a region (residues 654-665) of the al...Continue Reading

References

Nov 1, 1992·The Journal of Cell Biology·M P MarinkovichR E Burgeson
Jul 1, 1992·International Journal of Peptide and Protein Research·M NomizuP P Roller
Jun 1, 1992·The Journal of Cell Biology·P D YurchencoH Colognato
Dec 15, 1995·Journal of Molecular Biology·T SasakiR Timpl
Oct 1, 1995·The Journal of Investigative Dermatology·R FleischmajerM L Chu
Jun 1, 1995·The Journal of Investigative Dermatology·K B Yancey
Feb 1, 1995·Matrix Biology : Journal of the International Society for Matrix Biology·S M BernierY Yamada
Jan 20, 1995·Journal of Molecular Biology·T SasakiR Timpl
Oct 1, 1993·Clinics in Dermatology·F Ayala, P Santoianni
Nov 1, 1994·The Journal of Investigative Dermatology·R A EadyJ R McMillan
Apr 1, 1994·Experimental Cell Research·C M NiessenA Sonnenberg
Apr 26, 1994·Proceedings of the National Academy of Sciences of the United States of America·A M ChristianoJ Uitto

❮ Previous
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Citations

Oct 31, 2000·Microscopy Research and Technique·P TunggalM C Ott
Dec 18, 2001·International Journal of Cancer. Journal International Du Cancer·J Lohi
Jun 23, 2011·Journal of Cardiovascular Translational Research·Sonya B Seif-NaraghiKaren L Christman
Dec 4, 2003·Biochemical and Biophysical Research Communications·Annabelle CaptGuerrino Meneguzzi
Oct 18, 2001·Clinics in Dermatology·R F GhohestaniJ Uitto
May 3, 2003·Journal of Dermatological Science·James R McMillanHiroshi Shimizu
Apr 26, 2003·Matrix Biology : Journal of the International Society for Matrix Biology·Monique AumailleyLucy Tunggal
Jun 15, 1999·Matrix Biology : Journal of the International Society for Matrix Biology·M Aumailley, P Rousselle
Dec 22, 1999·Matrix Biology : Journal of the International Society for Matrix Biology·R GiltayG Kostka
Jul 17, 1999·European Journal of Biochemistry·S GrässelM L Chu
Oct 3, 1998·Journal of Anatomy·M Aumailley, N Smyth
Feb 24, 2011·Molecular & Cellular Proteomics : MCP·C Y X'avia ChanK W Michael Siu
Mar 29, 2014·Advances in Wound Care·Whitney M Longmate, C Michael Dipersio
Feb 14, 2014·Cellular and Molecular Life Sciences : CMLS·D OlijnykJ S Morris
Jun 18, 2005·Journal of Receptor and Signal Transduction Research·Yutaka MomotaAtsushi Utani
Apr 4, 2014·The Journal of Investigative Dermatology·Dara S MissanC Michael DiPersio
Mar 12, 2002·The Journal of Investigative Dermatology·B J DzambaL Y Sakai
Apr 26, 2007·The American Journal of Pathology·Chun-Hui YiMichael A Hollingsworth
Jul 8, 2009·International Journal of Cancer. Journal International Du Cancer·Sirpa SaloKarl Tryggvason
Mar 20, 2010·Birth Defects Research. Part C, Embryo Today : Reviews·Fenny WiradjajaIan Smyth
May 20, 2004·Experimental Cell Research·Abdallah ElkhalMonique Aumailley
Sep 27, 2006·European Journal of Cell Biology·Holm SchneiderFrederic Pacho
Dec 25, 2012·Cell Adhesion & Migration·Monique Aumailley
Jul 12, 2011·The American Journal of Pathology·Donald J AlcendorGary S Hayward
Jun 29, 2016·Cell and Tissue Research·C Michael DiPersioLivingston Van De Water
Sep 23, 2003·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·James R McMillanHiroshi Shimizu
Jun 5, 2003·Nature Reviews. Molecular Cell Biology·Rupert TimplMon-Li Chu
Jun 27, 2003·The Journal of Biological Chemistry·Atsushi UtaniHiroshi Shinkai
Apr 3, 2014·The Journal of Biological Chemistry·Yogita KananMuayyad R Al-Ubaidi
May 21, 2020·Molecular Medicine·Papiya ChakrabortyPranita P Sarangi
Feb 8, 2006·European Journal of Cell Biology·Michaela ZinnHans Smola

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