Fibulin-5 contributes to microfibril assembly in human periodontal ligament cells.

Acta Histochemica Et Cytochemica
Y HisanagaYoshihiko Sawa

Abstract

The elastic system fibers comprise oxytalan, elaunin and elastic fibers, which differ in their relative microfibril and elastin content. Human periodontal ligaments (PDL) contain only oxytalan fibers (pure microfibrils) among them. Since fibulin-5 regulates the organization of elastic fibers to link the fibers to cells, we hypothesized that fibulin-5 may contribute to the formation of oxytalan fibers. We used siRNA for fibulin-5 in PDL cell culture to examine the extracellular deposition of fibrillin-1 and -2, which are the major components of microfibrils. Fibulin-5 was labeled on microfibrils positive for fibrillin-1 and -2. Fibulin-5 suppression reduced the level of fibrillin-1 and -2 deposition to 60% of the control level. These results suggest that fibulin-5 may control the formation of oxytalan fibers, and play a role in the homeostasis of oxytalan fibers.

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Citations

May 27, 2011·Acta Histochemica Et Cytochemica·Takashi TaguchiMohammed S Razzaque
Feb 4, 2011·Histochemistry and Cell Biology·Martin GausterBerthold Huppertz
Jan 20, 2011·Acta Histochemica Et Cytochemica·Yoshinori YamauchiHiroyuki Ishikawa
Aug 13, 2010·Connective Tissue Research·Yuka NakatomiHiroyuki Ishikawa
Oct 24, 2013·Matrix Biology : Journal of the International Society for Matrix Biology·Mohamed A SideekMark A Gibson
Aug 8, 2015·Journal of Clinical Pathology·Jin Hyung HeoHee Jung An

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Methods Mentioned

BETA
electrophoresis
transfection
immunoprecipitation

Software Mentioned

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