PMID: 6159634Aug 1, 1980Paper

Fidelity of structure representation in electron micrographs of negatively stained protein molecules

Proceedings of the National Academy of Sciences of the United States of America
A C Steven, M A Navia

Abstract

We have investigated the fidelity of structure representation in electron micrographs of negatively stained proteins by conducting a systematic evaluation of such micrographs in terms of a known molecular structure, solved by x-ray crystallography. Microcrystals of immunoglobulin G Dob were used as specimens in this comparison between micrograph images, optimized by computer image processing, and reference images derived computationally from the crystal structure. To an effective resolution of 2 nm, we observed a remarkably good correlation between the experimental images and their idealized counterparts, which are unaffected by those factors--electron irradiation and dehydration--that are thought to be primarily responsible for perturbation of protein structure during electron microscopy. Separate structural features resolved in these micrographs do not, in general, correspond to specific components of individual molecules but arise instead from complex superpositions involving several overlapping molecules.

References

Jan 1, 1979·Annual Review of Biochemistry·L M Amzel, R J Poljak
Oct 25, 1979·Journal of Molecular Biology·S D FullerR Henderson
Nov 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·E W SilvertonD R Davies
Jan 1, 1979·Journal of Supramolecular Structure·A C Steven, J L Carrascosa
Jun 1, 1975·Journal of Ultrastructure Research·L W LabawD R Davies
Aug 1, 1972·Journal of Ultrastructure Research·L W Labaw, D R Davies
Oct 19, 1968·Nature·W D TerryD R Davies
Jan 1, 1973·Journal of Supramolecular Structure·U AebiE Kellenberger
Jul 1, 1959·Biochimica Et Biophysica Acta·S BRENNER, R W HORNE

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Citations

Aug 1, 1993·Molecular Immunology·R G DiScipio
Jul 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·A EngelW O Saxton
Oct 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·L M AmzelP L Pedersen
Jan 15, 1983·Virology·A C StevenB L Trus
Apr 23, 2008·Current Protocols in Protein Science·Alasdair Steven, David Belnap
Sep 1, 1986·Journal of Bacteriology·A C StevenJ L Cowell

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