Field application of immunoassays for the detection of Mycobacterium bovis infection in the African buffalo (Syncerus caffer)

Veterinary Immunology and Immunopathology
E M D L van der HeijdenA L Michel

Abstract

The African buffalo (Syncerus caffer) is considered the most important maintenance host of bovine tuberculosis (BTB) in wildlife in Southern Africa. The diagnosis of Mycobacterium bovis infection in this species mostly relies on the single intradermal comparative tuberculin test (SICTT). As an alternative, the BOVIGAM® 1G, an interferon-gamma (IFN-γ) release assay, is frequently used. The test performance of cell-mediated immunity (CMI-) and humoral immunity (HI-) based assays for the detection of M. bovis infections in buffaloes was compared to identify the test or test combination that provided the highest sensitivity in the study. Buffaloes were sampled during the annual BTB SICTT testing in the Hluhluwe-iMfolozi-Park (KwaZulu-Natal, South Africa) during June 2013. A total of 35 animals were subjected to the SICTT, 13 of these tested positive and one showed an inconclusive reaction. CMI-based assays (BOVIGAM® 1G (B1G) and BOVIGAM® 2G (B2G)) as well as a serological assay (IDEXX TB ELISA) were used to further investigate and compare immune responsiveness. Thirteen SICTT positive buffaloes and one inconclusive reactor were slaughtered and a post-mortem (PM) examination was conducted to confirm BTB. Lesions characteristic of BT...Continue Reading

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Citations

May 30, 2020·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Beibei JiaIan A Gardner
Dec 14, 2018·Frontiers in Veterinary Science·Christina MeiringWynand J Goosen
Nov 14, 2019·Comparative Immunology, Microbiology and Infectious Diseases·Elisabeth M D L van der HeijdenAnita L Michel
Feb 16, 2021·Frontiers in Veterinary Science·Netanya BernitzMichele A Miller
Feb 26, 2021·Veterinary Research·Jobin ThomasMaría A Risalde
Jan 24, 2020·Comparative Immunology, Microbiology and Infectious Diseases·Konstantin P LyashchenkoW Ray Waters

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