Field assessment of horse-associated genetic markers HoF597 and mtCytb for detecting the source of contamination in surface waters

Canadian Journal of Microbiology
Jessica GrayMohammad Katouli

Abstract

We investigated the specificity and sensitivity of two horse-associated markers, HoF597 and Horse mtCytb, and 12 mitochondrial and bacterial markers of six animal species (human, cow, pig, bird, dog, chicken) in the faecal samples of 50 individual horses. Both horse markers were detected in 48 (96%) faecal samples. Cross-reactivity with dog (BacCan545) and pig (P23-2) occurred in 88% and 72% of horse faecal samples, respectively. Several other bacterial and mitochondrial markers of non-target hosts were also detected; however, their specificities were >80%. Analyses of samples from surface waters (n = 11) on or adjacent to properties from which horse faecal samples had been collected showed only the presence of HoF597 but not horse mitochondrial marker. Our data suggest that while bacterial and (or) mitochondrial markers of other animal species may be present in horse faeces, dog and pig markers may predominantly be present in horse faecal samples, which points to their nonspecificity as markers for microbial source tracking. Although HoF597 and Horse mtCytb are highly sensitive and specific for the detection of horse faecal pollution, because of their low numbers, mitochondrial (mtDNA) markers may not be robust for screening s...Continue Reading

References

Jun 4, 2005·Applied and Environmental Microbiology·Linda K DickKatharine G Field
Jun 2, 2007·Environmental Science & Technology·Jane M CaldwellJay F Levine
Jun 26, 2007·Applied and Environmental Microbiology·Jennifer A UfnarR D Ellender
Aug 30, 2008·Water Science and Technology : a Journal of the International Association on Water Pollution Research·W AhmedT Gardner
Jan 13, 2009·Journal of Microbiological Methods·Jane M Caldwell, Jay F Levine
Jan 9, 2010·Water Science and Technology : a Journal of the International Association on Water Pollution Research·Craig Baker-AustinDavid N Lees
Jan 7, 2011·Applied Microbiology and Biotechnology·Peter Roslev, Annette S Bukh
Nov 15, 2011·Applied and Environmental Microbiology·Hyatt C GreenKatharine G Field
Apr 2, 2013·Canadian Journal of Microbiology·Corinne A KrentzJudith Isaac-Renton
Jul 25, 2013·Water Research·Alexandria B BoehmStephen B Weisberg
Jul 15, 2015·The Science of the Total Environment·Xiwei HeXu-Xiang Zhang

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