PMID: 9431934Feb 12, 1998Paper

Field evaluation of a combination of monospecific enzyme-linked immunosorbent assays for type-specific diagnosis of human immunodeficiency virus type 1 (HIV-1) and HIV-2 infections in HIV-seropositive persons in Abidjan, Ivory Coast

Journal of Clinical Microbiology
John N NkengasongA E Greenberg

Abstract

Serologic distinction between human immunodeficiency virus type 1 (HIV-1) and HIV-2 infection is made difficult because of the cross-reactivity and high cost of existing differentiation assays. An evaluation of a strategy based on a combination of monospecific enzyme-linked immunosorbent assays (ELISAs) (CME), was carried out in Abidjan, Ivory Coast, where both HIV-1 and HIV-2 are present, to determine its accuracy and cost-effectiveness. A total of 1,608 (428 HIV-1-positive, 361 HIV-2-positive, 371 dually HIV-1 and HIV-2 [HIV-D] reactive, and 448 HIV-negative) sera that had been serotyped by a line immunoassay (Peptilav) were tested retrospectively by an HIV-1-monospecific (Wellcozyme HIV Recombinant ELISA) and an HIV-2-monospecific (ICE*-HIV-2) assay. The CME strategy gave concordant results for all of the 428 sera scored as HIV-1 by Peptilav. Of the 361 sera scored as HIV-2 by Peptilav, 316 (87.5%) were scored as HIV-2 by CME; the remaining 45 sera were positive by both monospecific ELISAs (mean optical density ratios, 1.36 for Wellcozyme and 11.30 for ICE*-HIV-2) and were classified as HIV-D by CME. Of the 371 sera classified as HIV-D by Peptilav, 344 (92.7%), 21, and 6 were scored as HIV-D, HIV-1, and HIV-2, respectively, ...Continue Reading

References

Feb 1, 1992·Journal of Virological Methods·J NkengasongG van der Groen
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Jul 20, 1996·Lancet·L Gürtler
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Citations

Jun 8, 2001·Journal of Acquired Immune Deficiency Syndromes : JAIDS·C AdjéUNKNOWN UNAIDS HIV Drug Access Initiative, Abidjan, Côte d'Ivoire
Apr 10, 2002·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·T L ChorbaG Djomand
Aug 19, 2010·BMC Infectious Diseases·Georgette Adjorlolo-JohnsonTerence L Chorba
Apr 25, 2009·AIDS Research and Human Retroviruses·Debra L HansonJohn N Nkengasong
Aug 29, 2013·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Bo Langhoff HøngeUNKNOWN Bissau HIV Cohort Study Group
Apr 15, 2011·AIDS Research and Treatment·Kevin PetersonThushan I de Silva
Mar 25, 2018·HIV Medicine·B L HøngeUNKNOWN Bissau HIV Cohort study group
Mar 1, 2012·Indian Journal of Medical Microbiology·R KannangaiG Sridharan
Jun 22, 2001·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·R KannangaiG Sridharan
Dec 2, 2004·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Ebi C BiléJohn N Nkengasong

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