Field-portable wide-field microscopy of dense samples using multi-height pixel super-resolution based lensfree imaging

Lab on a Chip
Alon GreenbaumAydogan Ozcan

Abstract

We report a field-portable lensfree microscope that can image dense and connected specimens with sub-micron resolution over a large field-of-view of ~30 mm(2) (i.e., ~6.4 mm × ~4.6 mm) using pixel super-resolution and iterative phase recovery techniques. Weighing ~122 grams with dimensions of 4 cm × 4 cm × 15 cm, this microscope records lensfree in-line holograms of specimens onto an opto-electronic sensor-array using partially coherent illumination. To reconstruct the phase and amplitude images of dense samples (with >0.3 billion pixels in each image, i.e., >0.6 billion pixels total), we employ a multi-height imaging approach, where by using a mechanical interface the sensor-to-sample distance is dynamically changed by random discrete steps of e.g., ~10 to 80 μm. By digitally propagating back and forth between these multi-height super-resolved holograms (corresponding to typically 2-5 planes), phase and amplitude images of dense samples can be recovered without the need for any spatial masks or filtering. We demonstrate the performance of this field-portable multi-height lensfree microscope by imaging Papanicolaou smears (also known as Pap tests). Our results reveal the promising potential of this multi-height lensfree computa...Continue Reading

References

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Citations

Dec 14, 2012·Journal of Biomedical Optics·John WeidlingElliot Botvinick
Oct 3, 2013·PloS One·Alon GreenbaumAydogan Ozcan
Jun 24, 2014·Lab on a Chip·Zachary J SmithSebastian Wachsmann-Hogiu
Mar 13, 2013·The American Journal of Tropical Medicine and Hygiene·Isaac I BogochJennifer Keiser
Jun 1, 2013·Games for Health Journal·Sam MavandadiAydogan Ozcan
Sep 24, 2013·Lab on a Chip·Frederik ColleJurgen Del-Favero
Mar 19, 2013·Optics Letters·Marcella MatrecanoPietro Ferraro
Jul 1, 2014·ACS Nano·Euan McLeodAydogan Ozcan
Mar 12, 2016·Scientific Reports·Wei LuoAydogan Ozcan
Apr 24, 2013·Scientific Reports·Qingshan WeiAydogan Ozcan
Jan 21, 2014·Scientific Reports·Ikbal SencanAydogan Ozcan
May 17, 2012·Journal of Biophotonics·Guoan Zheng
Apr 6, 2013·IEEE Reviews in Biomedical Engineering·Zoltán Göröcs, Aydogan Ozcan
May 20, 2015·Virchows Archiv : an International Journal of Pathology·Yibo ZhangAydogan Ozcan
Jun 6, 2013·Optics Express·Alon GreenbaumAydogan Ozcan
Feb 4, 2014·Current Opinion in Biotechnology·Ahmet F Coskun, Aydogan Ozcan
Oct 10, 2012·Lab on a Chip·Hongying ZhuAydogan Ozcan
Dec 19, 2014·Science Translational Medicine·Alon GreenbaumAydogan Ozcan
Jul 16, 2016·Annual Review of Biomedical Engineering·Aydogan Ozcan, Euan McLeod
Aug 10, 2016·Biosensors & Bioelectronics·Mohendra RoySungkyu Seo
Nov 13, 2012·Analytical Chemistry·Feng GuoTony Jun Huang
Dec 3, 2016·PloS One·Victoria NguyenBabak Sanii
Sep 24, 2014·The American Journal of Tropical Medicine and Hygiene·Isaac I BogochJennifer Keiser
Jul 17, 2019·Journal of Biophotonics·Tairan LiuAydogan Ozcan
May 24, 2016·Reports on Progress in Physics·Euan McLeod, Aydogan Ozcan
Dec 3, 2016·Scientific Reports·Yair RivensonAydogan Ozcan
Nov 2, 2019·Journal of the Optical Society of America. A, Optics, Image Science, and Vision·Daniel DahanZeev Zalevsky
Aug 24, 2018·JCI Insight·Yichen DingTzung K Hsiai
Mar 10, 2019·Scientific Reports·Tairan LiuAydogan Ozcan
Apr 8, 2016·Light, Science & Applications·Wei LuoAydogan Ozcan

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