Field survey of sex-reversals in the medaka, Oryzias latipes: genotypic sexing of wild populations

Zoological Science
A ShinomiyaMitsuru Sakaizumi

Abstract

The medaka, Oryzias latipes, has an XX/XY sex determination mechanism. A Y-linked DM domain gene, DMY, has been isolated by positional cloning as a prime candidate for the sex-determining gene. Furthermore, the crucial role of DMY during male development was established by studying two wild-derived XY female mutants. In this study, to find new DMY and sex-determination related gene mutations, we conducted a broad survey of the genotypic sex (DMY-negative or DMY-positive) of wild fish. We examined 2274 wild-caught fish from 40 localities throughout Japan, and 730 fish from 69 wild stocks from Japan, Korea, China, and Taiwan. The phenotypic sex type agreed with the genotypic sex of most fish, while 26 DMY-positive (XY) females and 15 DMY-negative (XX) males were found from 13 and 8 localities, respectively. Sixteen XY sex-reversals from 11 localities were mated with XY males of inbred strains, and the genotypic and phenotypic sexes of the F(1) progeny were analyzed. All these XY sex-reversals produced XY females in the F(1) generation, and all F(1) XY females had the maternal Y chromosome. These results show that DMY is a common sex-determining gene in wild populations of O. latipes and that all XY sex-reversals investigated had ...Continue Reading

Citations

Dec 22, 2011·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Haruo MasuyamaMasaru Matsuda
Jun 21, 2005·Biochemical and Biophysical Research Communications·Masatoshi NakamotoNaoki Shibata
Apr 29, 2010·Heredity·A ShinomiyaM Sakaizumi
May 27, 2010·Development, Growth & Differentiation·Masatoshi NakamotoNaoki Shibata
Sep 1, 2007·BMC Developmental Biology·Amaury HerpinManfred Schartl
May 17, 2006·Genetics·Hiroyuki OtakeMitsuru Sakaizumi
Apr 9, 2008·Zoological Science·Tadashi SatoSatoshi Hamaguchi
Jul 12, 2014·Ecotoxicology·Catis Hin Ying CheungRudolf Shiu Sun Wu
Jan 16, 2010·Ecotoxicology and Environmental Safety·Kevin FlynnRodney Johnson
Sep 27, 2015·Biochemical and Biophysical Research Communications·Takuto ImaiMasaru Matsuda
Apr 25, 2006·Biochemical and Biophysical Research Communications·Masatoshi NakamotoNaoki Shibata
Nov 26, 2015·Zoological Letters·Maiko KawajiriKazunori Yamahira
Nov 16, 2005·Annual Review of Genetics·Masaru Matsuda
Sep 24, 2004·Current Biology : CB·Mariko KondoManfred Schartl
Oct 10, 2015·General and Comparative Endocrinology·Masaru Matsuda, Mitsuru Sakaizumi
Jul 28, 2016·Journal of Applied Toxicology : JAT·Yoshifumi HorieNorihisa Tatarazako
Dec 3, 2016·Sexual Development : Genetics, Molecular Biology, Evolution, Endocrinology, Embryology, and Pathology of Sex Determination and Differentiation·Jean-François Baroiller, Helena D'Cotta
Aug 31, 2006·Journal of Experimental Zoology. Part A, Comparative Experimental Biology·Ai ShinomiyaSatoshi Hamaguchi
Mar 8, 2007·Molecular Reproduction and Development·Masatoshi NakamotoNaoki Shibata
Apr 25, 2019·Environmental Toxicology and Chemistry·Ellen MihaichJeffrey Wolf
Feb 1, 2009·Current Protocols in Toxicology·Stephanie PadillaDoris W T Au
Jul 18, 2017·Molecular and Cellular Endocrinology·Masatoshi NakamotoYoshitaka Nagahama

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Methods Mentioned

BETA
PCR
electrophoresis

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