Fifteen microsatellite markers for Herbertia zebrina (Iridaceae): An endangered species from South American grasslands

Applications in Plant Sciences
Cristiane ForgiariniTatiana Teixeira de Souza-Chies

Abstract

Polymorphic microsatellite loci were developed and used to genotype individuals of Herbertia zebrina (Iridaceae) as a first step for assessment of intraspecific genetic diversity. Primer pairs for 47 markers were developed: 20 from a microsatellite-enriched library and 27 from a next-generation sequencing run using the Illumina MiSeq platform. Of those, 15 loci were considered successful, of which 12 were polymorphic and three were monomorphic. The primers were tested in 50 individuals from three populations of H. zebrina. Two to 14 alleles per locus were identified, and observed and expected heterozygosity were 0.00-0.95 and 0.18-0.89, respectively. Tests of cross-amplification to evaluate the applicability of these markers showed positive results in one congeneric species, H. darwinii, and in a phylogenetically closely related species, Calydorea crocoides. These microsatellite markers can be used for studies of genetic variation and genetic population structure, as well as to support conservation efforts.

References

Feb 5, 2000·Nature Biotechnology·M Schuelke
Jan 1, 2008·Molecular Ecology Resources·François Rousset
Jun 26, 2012·Nucleic Acids Research·Andreas UntergasserSteven G Rozen

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Methods Mentioned

BETA
dot blot
PCR
electrophoresis
genotyping

Software Mentioned

Primer3Plus
MICRO
CHECKER
Primer3
GENEPOP
GeneMarker
R

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