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Filamentation and restoration of normal growth in Escherichia coli using a combined CRISPRi sgRNA/antisense RNA approach

PloS One

Sep 11, 2018

Andrea MücklFriedrich Christian Simmel

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Abstract

CRISPR interference (CRISPRi) using dCas9-sgRNA is a powerful tool for the exploration and manipulation of gene functions. Here we quantify the reversible switching of a central process of the bacterial cell cycle by CRISPRi and an antisense RNA mechanism. Reversible induction of filame...read more

Mentioned in this Paper

Alkalescens-Dispar Group
Bacterial Proteins
Cell Cycle
Cell Division
Cytoskeletal Proteins
Escherichia coli
Induction, Genetic
Metabolic Inhibition
Antisense RNA
RNA, Guide
2
19
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Filamentation and restoration of normal growth in Escherichia coli using a combined CRISPRi sgRNA/antisense RNA approach

PloS One

Sep 11, 2018

Andrea MücklFriedrich Christian Simmel

PMID: 30204770

DOI: 10.1371/journal.pone.0198058

Abstract

CRISPR interference (CRISPRi) using dCas9-sgRNA is a powerful tool for the exploration and manipulation of gene functions. Here we quantify the reversible switching of a central process of the bacterial cell cycle by CRISPRi and an antisense RNA mechanism. Reversible induction of filame...read more

Mentioned in this Paper

Alkalescens-Dispar Group
Bacterial Proteins
Cell Cycle
Cell Division
Cytoskeletal Proteins
2
19

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