PMID: 7525606Dec 1, 1994Paper

Filipin-sensitive caveolae-mediated transport in endothelium: reduced transcytosis, scavenger endocytosis, and capillary permeability of select macromolecules

The Journal of Cell Biology
J E SchnitzerJ Allard

Abstract

Caveolae or noncoated plasmalemmal vesicles found in a variety of cells have been implicated in a number of important cellular functions including endocytosis, transcytosis, and potocytosis. Their function in transport across endothelium has been especially controversial, at least in part because there has not been any way to selectively inhibit this putative pathway. We now show that the ability of sterol binding agents such as filipin to disassemble endothelial noncoated but not coated plasmalemmal vesicles selectively inhibits caveolae-mediated intracellular and transcellular transport of select macromolecules in endothelium. Filipin significantly reduces the transcellular transport of insulin and albumin across cultured endothelial cell monolayers. Rat lung microvascular permeability to albumin in situ is significantly decreased after filipin perfusion. Conversely, paracellular transport of the small solute inulin is not inhibited in vitro or in situ. In addition, we show that caveolae mediate the scavenger endocytosis of conformationally modified albumins for delivery to endosomes and lysosomes for degradation. This intracellular transport is inhibited by filipin both in vitro and in situ. Other sterol binding agents inclu...Continue Reading

References

Jan 1, 1977·Annual Review of Biochemistry·S C SilversteinZ A Cohn
Jan 1, 1992·Tissue & Cell·R L Roberts, A Sandra
May 1, 1992·The Journal of Cell Biology·L Ghitescu, M Bendayan
Feb 21, 1992·Cell·K G RothbergR G Anderson
Nov 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·D TranL Orci
Dec 1, 1989·The Journal of Cell Biology·J KartenbeckA Helenius
Apr 6, 1989·The New England Journal of Medicine·D SteinbergJ L Witztum
Jul 1, 1988·The Journal of Cell Biology·N GhineaN Simionescu
Jan 1, 1985·Annual Review of Cell Biology·J L GoldsteinW J Schneider
Dec 1, 1987·The Journal of Cell Biology·A J MiliciG E Palade
Sep 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·J E SchnitzerG E Palade
Mar 29, 1985·Science·G L King, S M Johnson
Nov 8, 1984·Nature·W A JefferiesD Y Mason
Jan 1, 1983·Annual Review of Biochemistry·M S Brown, J L Goldstein
Dec 1, 1984·Journal of Ultrastructure Research·J S Geoffroy, R P Becker
May 1, 1983·Microvascular Research·R C WagnerJ J Devenny
Jul 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·M S BretscherB M Pearse
Mar 25, 1993·Nature·T SöllnerJ E Rothman
Jun 1, 1968·The Journal of Cell Biology·G E Palade, R R Bruns
Sep 25, 1955·The Journal of Biophysical and Biochemical Cytology·E YAMADA

❮ Previous
Next ❯

Citations

Jul 9, 1999·Reviews in Medical Virology·N E Bishop
May 29, 1999·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·A PolC Enrich
Sep 25, 2002·Journal of Neuroscience Research·Anuska V AndjelkovicJoel S Pachter
Jul 20, 2006·Acta Neuropathologica·V BarresiG Barresi
May 13, 2005·Histochemistry and Cell Biology·Martin WestermannWalter Richter
May 1, 2012·Histochemistry and Cell Biology·Monika SramkovaRoberto Weigert
Oct 7, 2008·Cell and Tissue Research·Maya SimionescuAnca Sima
Jan 19, 2010·Cellular and Molecular Neurobiology·Ping WangYunhui Liu
Sep 29, 2011·Molecular and Cellular Biochemistry·Tarun E HutchinsonJawaharlal M Patel
Apr 17, 2013·Reviews in Endocrine & Metabolic Disorders·Tetsuya KubotaTakashi Kadowaki
Dec 11, 2008·Pathology Oncology Research : POR·Anna L Kiss, Erzsébet Botos
Aug 1, 1995·Current Opinion in Cell Biology·C Lamaze, S L Schmid
Apr 28, 2004·The International Journal of Biochemistry & Cell Biology·Nicole S Harhaj, David A Antonetti
Dec 23, 2003·The International Journal of Biochemistry & Cell Biology·Elena S KuzmenkoKlaus Fiedler
May 8, 2013·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Chao ZhengXinge Zhang
Jul 27, 2001·Mechanisms of Ageing and Development·T FülöpG Dupuis
Jul 11, 2003·Placenta·E A LintonC W G Redman
Mar 20, 2003·Brain Research. Developmental Brain Research·Dong Hoon ShinWang Jae Lee
Mar 6, 2003·Journal of Controlled Release : Official Journal of the Controlled Release Society·Mark GumbletonGlyn Taylor
Sep 12, 2001·Advanced Drug Delivery Reviews·S MatveevE J Smart
Jun 6, 2000·Advanced Drug Delivery Reviews·O V GerasimovD H Thompson
May 5, 1998·Cancer Letters·A R GagliardiD C Collins
Aug 1, 1996·Current Opinion in Cell Biology·R G Parton
Aug 1, 1997·Current Opinion in Cell Biology·T Harder, K Simons
Apr 2, 2003·The Journal of Nutritional Biochemistry·Michael F MouatArthur Grider
Dec 24, 2002·Trends in Cell Biology·David L Marks, Richard E Pagano
Jul 28, 2001·Micron : the International Research and Review Journal for Microscopy·A L KissE Botos
Jul 10, 2003·Lancet Neurology·Eain M Cornford, Marcia E Cornford
Jan 18, 2003·Vascular Pharmacology·Michel DemeuleRichard Béliveau
Feb 4, 2010·Cell Research·Sudha KumariSatyajit Mayor

❮ Previous
Next ❯

Related Concepts

Related Feeds

Caveolins & Signal Transduction

Caveolins are small proteins with a hairpin loop conformation that are located in the plasma membrane of various cell types where they bind cholesterol and interact with receptors essential for several signal transduction pathways. Here is the latest research.