Filling the Gaps - A Call for Comprehensive Analysis of Extracellular Matrix of the Glial Scar in Region- and Injury-Specific Contexts

Frontiers in Cellular Neuroscience
Jacob Kjell, Magdalena Götz

Abstract

Central nervous system (CNS) injury results in chronic scar formation that interferes with function and inhibits repair. Extracellular matrix (ECM) is prominent in the scar and potently regulates cell behavior. However, comprehensive information about the ECM proteome is largely lacking, and region- as well as injury-specific differences are often not taken into account. These aspects are the focus of our perspective on injury and scar formation. To highlight the importance of such comprehensive proteome analysis we include data obtained with novel analysis tools of the ECM composition in the scar and show the contribution of monocytes to the ECM composition after traumatic brain injury (TBI). Monocyte invasion was reduced using the CCR2-/- mouse line and step-wise de-cellularization and proteomics allowed determining monocyte-dependent ECM composition and architecture of the glial scar. We find significant reduction in the ECM proteins Tgm1, Itih (1,2, and 3), and Ftl in the absence of monocyte invasion. We also describe the scar ECM comprising zones with distinctive composition and show a subacute signature upon comparison to proteome obtained at earlier times after TBI. These results are discussed in light of injury-, region...Continue Reading

References

Jan 20, 1997·The Journal of Comparative Neurology·P G PopovichB T Stokes
Apr 12, 2002·Nature·Elizabeth J BradburyStephen B McMahon
Mar 3, 2007·Stroke; a Journal of Cerebral Circulation·Oliver B DimitrijevicAnuska V Andjelkovic
Jun 23, 2010·Brain : a Journal of Neurology·Daniela CarulliJames W Fawcett
Jul 9, 2011·Science·Christian GöritzJonas Frisén
Oct 22, 2011·Biochimica Et Biophysica Acta·Soner GundemirGail V W Johnson
Dec 14, 2011·Molecular & Cellular Proteomics : MCP·Alexandra NabaRichard O Hynes
Jun 22, 2012·Annals of Neurology·Michael GliemSebastian Jander
Dec 4, 2012·Nucleic Acids Research·Juan Antonio VizcaínoHenning Hermjakob
Feb 27, 2013·Molecular Cell·Matthias MannJürgen Cox
Aug 24, 2013·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Cynthia SoderblomJae K Lee
Nov 26, 2013·Current Biology : CB·Stephanie MajkutDennis E Discher
Jan 16, 2014·Experimental Neurology·Jared M CreggJerry Silver
Apr 3, 2014·Physiological Reviews·Richard L EckertKapil Mehta
Apr 4, 2014·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Katalin BartusElizabeth J Bradbury
Sep 6, 2014·Frontiers in Cellular Neuroscience·Lars Roll, Andreas Faissner
Jan 16, 2015·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Josh M MorgantiSusanna Rosi
Mar 6, 2015·EMBO Reports·Caghan KizilMichael Brand
Jul 16, 2015·Molecular Systems Biology·Herbert B SchillerMatthias Mann
Dec 30, 2015·Nature Neuroscience·Stefanie Robel, Harald Sontheimer
Apr 15, 2016·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Somsak WattananitZaal Kokaia
May 3, 2016·Cell Systems·Philipp E GeyerMatthias Mann
Jun 28, 2016·Nature Methods·Stefka TyanovaJürgen Cox
Oct 14, 2016·Disease Models & Mechanisms·Jacob Kjell, Lars Olson
Jan 19, 2017·Nature·Shane A LiddelowBen A Barres
Mar 21, 2017·Nature Communications·Emad MoeendarbaryKristian Franze
Dec 23, 2017·Nature Neuroscience·Katrina L Adams, Vittorio Gallo
May 3, 2018·Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics·Michael B Orr, John C Gensel
May 15, 2018·International Journal of Molecular Sciences·Jure TicaAthanasios Didangelos
May 18, 2018·Nature·Roger A BarkerMalin Parmar
Oct 6, 2018·Seminars in Cell & Developmental Biology·Lars Roll, Andreas Faissner
Mar 1, 2019·Nature Communications·Ilias AngelidisHerbert B Schiller

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Citations

Dec 29, 2020·Progress in Neurobiology·Francina Mesquida-VenyArnau Hervera

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Datasets Mentioned

BETA
PXD017478

Methods Mentioned

BETA
solubility profiling

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