Film from peroxidation of an amino phospholipid and its biocompatibility

Journal of Biomedical Materials Research. Part B, Applied Biomaterials
Hua-Jie WangJin-Ye Wang

Abstract

A new kind of phospholipid product with fluorescence was derived from autoxidation of an aminophospholipid, L-alpha-phosphatidylethanolamine dilinoleoyl in solid film state. Atomic force microscopy showed that the film product was composed of round and elliptical vesicles with diameters of about 20-45 nm. The product was difficult to dissolve in water and most organic solvents. Fourier transform infrared spectroscopy, solid-state (13)C NMR, and solid-state (31)P NMR indicated that the unsaturated double carbon bond decreased with the reaction, and polymerization within/between phospholipid molecules occurred. Endothelial cells from human vein seeded onto the film showed the highest proliferating activity compared with seeding onto Corning culture plate, glass plate, or phospholipid film with phosphorylcholine head group, as evaluated by 3-(4,5-timethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay (p < 0.001). The hemocompatibility of the film was also assessed by platelet adhesion and protein adsorption. The results suggest that the product has potential applications as a new biomaterial coating.

References

Feb 1, 1994·Analytical Biochemistry·P BellaviteC Santonastaso
Jul 9, 1999·Journal of Biomedical Materials Research·W B TsaiT A Horbett
Dec 11, 1999·Journal of Biomaterials Science. Polymer Edition·K IshiharaN Nakabayashi
Jul 18, 2001·Biomaterials·M RiedelE Wintermantel
Jul 18, 2002·Proceedings of the National Academy of Sciences of the United States of America·Julia J HwangSamuel I Stupp
Aug 23, 2002·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Andrea GessnerRainer Müller
Apr 19, 2003·Carbohydrate Research·Varawut TangpasuthadolVipavee P Hoven
May 8, 2003·Biological Procedures Online·Aleksandra SklodowakaRenata Matlakowska
May 17, 2005·Journal of Controlled Release : Official Journal of the Controlled Release Society·Hua-Jie WangJin-Ye Wang

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