PMID: 8177830Dec 1, 1993Paper

Filtration and recirculation of early amniotic fluid. Evaluation of cell cultures from 100 diagnostic cases

Prenatal Diagnosis
K SundbergJ Philip

Abstract

Due to the low cell concentration, cultures from early amniotic fluid specimens usually require 2-3 weeks in culture prior to karyotyping. The purpose of this study was to evaluate the culture quality of amniotic fluid cells from early pregnancy, obtained by a new filter technique. The hypothetical advantage of the technique was that the increased cell yield might reduce the culture time before karyotyping. Culture quality was assessed by the number of colonies, the percentage of colonies containing mitoses in filter and control cultures, and the culture time. The setting was a consecutive clinical trial. One hundred samples were obtained from ongoing pregnancies at 11-14 weeks of gestation (mean 12.8 weeks). By circulating a mean of 26 ml of amniotic fluid through a cell filter system leading the cell-free fluid back to the amniotic cavity, the cell yield was increased in the sample of 7 ml corresponding to the dead space of the filter system. The culture results were compared with control cultures from 5 ml samples drawn from the same pregnancies prior to recirculation. The cultures from the first flushing of the filter system yielded 2.6 times more colonies and in total 4.2 times more colonies were found in the three culture...Continue Reading

References

Jan 1, 1977·Hereditas·W Schmid
Apr 1, 1992·Prenatal Diagnosis·F S JørgensenJ Philip
Jan 1, 1991·Fetal Diagnosis and Therapy·D ByrneK Nicolaides
Jun 1, 1990·Prenatal Diagnosis·L StripparoP De Biasio
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Jul 1, 1989·BMJ : British Medical Journal·D E RooneyD V Coleman
Jun 1, 1987·American Journal of Obstetrics and Gynecology·F W HansonS Samuels
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May 1, 1993·Seminars in Pediatric Surgery·J C Langer
Sep 1, 1991·Ultrasound in Obstetrics & Gynecology : the Official Journal of the International Society of Ultrasound in Obstetrics and Gynecology·D L ByrneK H Nicolaides
Jul 1, 1991·Ultrasound in Obstetrics & Gynecology : the Official Journal of the International Society of Ultrasound in Obstetrics and Gynecology·D ByrneK Nicolaides
Mar 1, 1991·Ultrasound in Obstetrics & Gynecology : the Official Journal of the International Society of Ultrasound in Obstetrics and Gynecology·K SundbergJ Philip

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Citations

Mar 26, 1994·Lancet·T BryndorfC Gaiser
Apr 1, 1996·British Journal of Obstetrics and Gynaecology·L EriksenK Sundberg
Jan 1, 1995·Journal of Perinatal Medicine·K SundbergJ Bang

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