Fimsbactin and Acinetobactin Compete for the Periplasmic Siderophore Binding Protein BauB in Pathogenic Acinetobacter baumannii

ACS Chemical Biology
Tabbetha J BohacTimothy A Wencewicz

Abstract

Environmental and pathogenic microbes produce siderophores as small iron-binding molecules to scavenge iron from natural environments. It is common for microbes to produce multiple siderophores to gain a competitive edge in mixed microbial environments. Strains of human pathogenic Acinetobacter baumannii produce up to three siderophores: acinetobactin, baumannoferrin, and fimsbactin. Production of acinetobactin and baumannoferrin is highly conserved among clinical isolates while fimsbactin production appears to be less common. Fimsbactin is structurally related to acinetobactin through the presence of catecholate and phenolate oxazoline metal-binding motifs, and both are derived from nonribosomal peptide assembly lines with similar catalytic domain orientations and identities. Here we report on the chemical, biochemical, and microbiological investigation of fimsbactin and acinetobactin alone and in combination. We show that fimsbactin forms a 1:1 complex with iron(III) that is thermodynamically more stable than the 2:1 acinetobactin ferric complex. Alone, both acinetobactin and fimsbactin stimulate A. baumannii growth, but in combination the two siderophores appear to compete and collectively inhibit bacterial growth. We show t...Continue Reading

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Citations

May 10, 2020·Biomolecules·Maria Soledad RamirezMarcelo E Tolmasky
Jul 10, 2019·Journal of Molecular Biology·Timothy A Wencewicz
Nov 5, 2019·Research in Microbiology·Philippe Delepelaire
Jun 11, 2021·ACS Infectious Diseases·Tabbetha J BohacTimothy A Wencewicz
Jun 30, 2019·Inorganic Chemistry·Elizabeth W Hunsaker, Katherine J Franz
Nov 6, 2021·Biochemistry·Adunoluwa O ObisesanElizabeth M Nolan
Dec 3, 2021·Mass Spectrometry Reviews·Rutuja H PatilVladimír Havlíček

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