Finding of Agr Phase Variants in Staphylococcus aureus

MBio
Vishal GorKazuya Morikawa

Abstract

Staphylococcus aureus is an important human pathogen whose success is largely attributed to its vast arsenal of virulence factors that facilitate its invasion into, and survival within, the human host. The expression of these virulence factors is controlled by the quorum sensing accessory gene regulator (Agr) system. However, a large proportion of clinical S. aureus isolates are consistently found to have a mutationally inactivated Agr system. These mutants have a survival advantage in the host but are considered irreversible mutants. Here we show, for the first time, that a fraction of Agr-negative mutants can revert their Agr activity. By serially passaging Agr-negative strains and screening for phenotypic reversion of hemolysis and subsequent sequencing, we identified two mutational events responsible for reversion: a genetic duplication plus inversion event and a poly(A) tract alteration. Additionally, we demonstrate that one clinical Agr-negative methicillin-resistant S. aureus (MRSA) isolate could reproducibly generate Agr-revertant colonies with a poly(A) tract genetic mechanism. We also show that these revertants activate their Agr system upon phagocytosis. We propose a model in which a minor fraction of Agr-negative S....Continue Reading

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Citations

Feb 6, 2020·Nucleic Acids Research·Pilar Menendez-GilAlejandro Toledo-Arana
Jan 29, 2021·Microorganisms·Vishal GorKazuya Morikawa
Feb 8, 2021·Cellular Microbiology·Ye Mon SoeAbderrahman Hachani
Jul 10, 2021·Wellcome Open Research·Fiona SargisonJ Ross Fitzgerald
May 26, 2021·Current Opinion in Microbiology·Dallas L Mould, Deborah A Hogan
Apr 7, 2021·Infection and Immunity·Roshika RoshikaPaul Sumby
Jul 15, 2021·FEMS Microbiology Reviews·Elisa J M RaineriJan Maarten van Dijl

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Methods Mentioned

BETA
fluorescence microscopy
genotyping
PCR

Software Mentioned

FSX
DNASTAR
BSW

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