PMID: 9164601May 15, 1997Paper

Fine epitope specificity of antibodies to region II of the Plasmodium vivax circumsporozoite protein correlates with ability to bind recombinant protein and sporozoites

Acta Tropica
J BilsboroughM F Good

Abstract

Recent work has suggested that important B- and T-cell epitopes on the circumsporozoite protein (CSP) of Plasmodium vivax lie external to the major repeat regions of the protein. We have studied two naturally exposed human populations (Caucasian and Papua New Guineans) and determined the antibody response to yeast-derived recombinant CSPs, overlapping synthetic peptides spanning amino acids 76 348 of the Belem P. vivax CSP and overlapping peptides representing the variant repeats of the VK247 strain of P. vivax. We have demonstrated that the P. vivax CSP-specific antibody response is directed towards areas within the repeat region as well as areas external to this; but the dominant epitopes recognized by the two populations studied, were distinct. One epitope, lying external to the repeats and recognized by both populations, partially overlaps an area of the protein referred to as region II-plus. Sera from malaria-exposed Papua New Guineans and Thais contained antibodies to this epitope (V22, single letter amino acid sequence TCGVGVRVRRRVNAANKKPE) which were capable of recognizing sporozoites, as determined by quantitative inhibition IFA. Seventeen percent of PNG sera had antibodies to this peptide compared with 33% who had ant...Continue Reading

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Citations

Mar 30, 2012·The Journal of Infectious Diseases·Anjali YadavaJoanne M Lumsden
Dec 3, 2003·Tropical Medicine & International Health : TM & IH·Kap No LeeChaeseung Lim
Jan 17, 2012·Proceedings of the National Academy of Sciences of the United States of America·James J MoonDarrell J Irvine
Jun 21, 2005·Microbiology and Immunology·Chae Seung LimYoung Chang Tockgo

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