Fine Mapping and Candidate Gene Identification for Wax Biosynthesis Locus, BoWax1 in Brassica oleracea L. var. capitata

Frontiers in Plant Science
Dongming LiuLimei Yang

Abstract

Cuticular waxes play important roles in plant protection against various biotic and abiotic environmental stresses. The cuticular wax covering gives normal cabbage a glaucous appearance, but the appearance of waxless mutant is glossy green. Based on the present study, inheritance of the glossy green character of mutant HUAYOU2 follows a simple recessive pattern. Genetic analysis of an F2 population comprising 808 recessive individuals derived from HUAYOU2 (P1, maternal parent) and M36 (P2, paternal parent) revealed that a single recessive locus, BoWax1 (Brassica oleracea Wax 1), controls glossy green trait in B. oleracea. This locus was mapped to a region of 158.5 kb on chromosome C01. Based on nucleotide sequence analysis, Bol013612 was identified as the candidate gene for BoWax1. Sequencing results demonstrated that there is a deletion mutation of two nucleotides in the cDNA of Bol013612 of HUAYOU2, which may account for its glossy green trait. These results lay the foundation for functional analysis of BoWax1 and may accelerate research on wax metabolism in cabbage.

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Citations

Oct 16, 2020·International Journal of Molecular Sciences·Magdalena GóralskaBeata Myśków
Jun 14, 2021·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Jie LiuTingdong Fu

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Datasets Mentioned

BETA
MG808275

Methods Mentioned

BETA
PCR

Software Mentioned

JoinMap
Multalin
Primer Premier
MapDraw

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