Fine Mapping and Candidate Gene Prediction for White Immature Fruit Skin in Cucumber (Cucumis sativus L.)

International Journal of Molecular Sciences
Hong-Yu TangZheng-Jie Wang

Abstract

In this study, a single recessive gene (designated w₀) was identified to control the white immature fruit color. Genetic mapping with simple sequence repeats (SSR) markers located the w₀ gene in the distal region of cucumber chromosome 3 (Chr.3). Fine mapping was then conducted using the method of draft genome scaffold-assisted chromosome walking with 7304 F₂ individuals, which allowed for the assignment of the gene locus to a 100.3 kb genomic DNA region with two flanking markers, Q138 and Q193. Thirteen candidate genes were predicted in the 100.3 kb region. Quantitative real-time polymerase chain reaction (qRT-PCR) analysis showed that the expression of the Csa3G904140 gene, which encodes a two-component response regulator-like protein, was much higher in the immature fruit skin of the green parental line (Q1) than in the white parental line (H4). A coding sequence analysis suggested that a single-base insertion occurred at the ninth exon, resulting in a frameshift mutation in Csa3G904140 of H4, and the mutation was consistent with the phenotype in 17 green/white germplasms. Therefore, Csa3G904140 was taken as the likely candidate gene controlling the immature fruit color of cultivated cucumber. This study will contribute to t...Continue Reading

References

Apr 12, 2000·The Plant Journal : for Cell and Molecular Biology·F E VaistijJ D Rochaix
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Mar 16, 2007·Plant Cell Reports·Eugene A Lysenko
Feb 19, 2008·Trends in Plant Science·Andrew C AllanWilliam A Laing
Mar 29, 2008·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Junping HeGuangsheng Yang
Oct 23, 2009·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Weiwei ZhangRun Cai
Mar 5, 2016·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Hanqiang LiuZhihui Cheng

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Citations

Jul 10, 2019·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Bingbing LiWenge Liu
Feb 24, 2020·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Soo Bin LeeJe Min Lee
Jun 29, 2021·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Kiros GebretsadikKailiang Bo

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Methods Mentioned

BETA
PCR
transmission electron microscopy

Software Mentioned

DNAMAN
BLAST

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