Fine mapping and functional characterization of two immuno-dominant regions from the preS2 sequence of hepatitis B virus

Intervirology
Helga MeiselE Grens

Abstract

A set of monoclonal antibodies (mAbs) directed against the preS2 region of hepatitis B virus (HBV) surface antigen (HBsAg) was generated by immunization of mice with native HBsAg isolated from the blood of HBV carriers. According to (1) mutual competition binding of mAb to natural HBsAg, (2) recognition of full-length preS2 displayed on hepatitis B core particles, (3) recognition of synthetic partial preS2 peptides, and (4) Western blotting using a fusion protein library of truncated preS2 fragments of different legths, mAbs were assigned to two groups which coincided with groups I and III described by Mimms et al. [Virology 1990; 176:604-619]. All mAbs recognized linear epitopes and were glycosylation independent. Six out of eight fine-mapped mAbs recognized common epitopes located in the amino-terminal part of the preS sequence between amino acids 131 and 144 (group I), and inhibited binding of HBsAg to polymerized human serum albumin. Only two mAbs recognized a carboxy-terminal HBV-genotype-specific epitope covering amino acid residues 162 to 168 (group III). These mAbs bound to the highly variable proteolysis-sensitive hinge of preS2. Although four out of six mAbs targeted to immunodominant region I require the full-length ...Continue Reading

Citations

Apr 16, 2002·Viral Immunology·A LundkvistR Ulrich
Sep 11, 2014·World Journal of Gastroenterology : WJG·Wen Siang Tan, Kok Lian Ho
Oct 9, 2007·Journal of Peptide Science : an Official Publication of the European Peptide Society·Min LianXiaofeng Zheng

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